Separation and estimation of seven vasodilators using packed column supercritical fluid chromatography

Bhoir, Indravadan C; Raman, Bhanu; Sundaresan, M.; Bhagwat, A. M.

doi:10.1016/s0731-7085(97)00187-8

Cited by 16 publications

(7 citation statements)

References 17 publications

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“…These include SFC method [5], HPTLC method [6], HPLC reverse phase methods using various particle size columns and buffers [7][8][9][10]. The drug was also studied extensively for pharmacokinetics and metabolism in animals and human subjects leading to development of a number of HPLC methods using UV and mass detection [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Degradation studies of pentoxifylline: Isolation and characterization of a novel gem-dihydroperoxide derivative as major oxidative degradation product

Mone¹,

Chandrasekhar²

2010

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Degradation studies of pentoxifylline: Isolation and characterization of a novel gem-dihydroperoxide derivative as major oxidative degradation product

Mone¹,

Chandrasekhar²

2010

Journal of Pharmaceutical and Biomedical Analysis

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“…Nonvolatile, thermally and chemically labile drug molecules can be safely analyzed by SFC. Estimation of Prostaglandins (Koski and Jansson 1991), combination of non-steroidal anti-inflammatory drugs (Jagota and Stewart 1992), anticancer drugs (Musser and Callery 1990), antipsychotics (Berger and Wilson 1994), vasodilators (Bhoir et al 1998) and estimation of several other drugs from blood can be done by standard SFC procedures.…”

Section: Industrial Applications Of Scfs and Sfcsmentioning

confidence: 99%

Supercritical Fluid Chromatography: A Green Approach for Separation and Purification of Organic and Inorganic Analytes

Datta

Auddy

2013

Green Chromatographic Techniques

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'Supercritical fluid' describes a gas or liquid at conditions above its critical point. A greater range of solvent properties can be achieved with Supercritical fluid as a single solvent by careful manipulation of temperature and pressure at the supercritical state. Supercritical fluids are attractive media for several chemical reactions having better control over the reaction rates in different areas of biochemistry, polymer chemistry and environmental science. Supercritical fluid extraction (SFE), a rapid, convenient, efficient, and selective method has been used successfully for the separation of analytes prior to supercritical fluid chromatography (SFC), which is a relatively recent chromatographic technique and is commercially available since 1982. SFC significantly reduces the usage of organic solvents and wastes by using supercritical CO 2 as the mobile phase. The important principles of green chemistry that are applicable to green chromatography includes prevention of waste, use of safer solvents and increasing energy efficiency. All these factors are taken care of in SFC which combines some of the best features of HPLC as well as GC. Analytes that cannot be vaporized for analysis by gas chromatography or have no functional groups for detection by the usual liquid chromatography techniques, can be separated and detected using SFC. By now SFC has been applied to wide variety of materials including natural products, pharmaceuticals, foods, pesticides, herbicides, surfactants, polymers and polymer additives, heavy metals, fossils fuels, petroleum, explosives and propellants. SFC has now become an attractive alternative for chiral drug separation.

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“…A detailed survey of the literature for AM reveals several methods based on different techniques, viz. HPLC [5], high performance thin layer chromatography [6], over pressured liquid chromatography [7], supercritical fluid chromatography [8], and UV spectrophotometry [9] for its determination in pharmaceutical dosage forms. Similarly, a literature survey for AT calcium reveals methods based on HPLC [10], CE [11], and UV spectrophotometry [12] for its determination in pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

An improved HPLC method with the aid of a chemometric protocol: Simultaneous analysis of amlodipine and atorvastatin in pharmaceutical formulations

Sivakumar

Manavalan

Muralidharan

et al. 2007

J of Separation Science

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Statistical experimental design and Derringer's desirability function were applied to develop an improved RP-HPLC method for the simultaneous analysis of amlodipine and atorvastatin in pharmaceutical formulations. Four independent factors were considered: acetonitrile content in the mobile phase; buffer pH; buffer concentration; and flow rate. The preliminary screening step was carried out, according to a 2(4-1) fractional factorial design, to identify the significant factors affecting the analysis time response. Then central composite design was applied for a response surface study, in order to examine in depth the effects of the most important factors. Subsequently, Derringer's desirability function was employed to simultaneously optimize the six responses: retention factor of first peak; two resolutions; and three retention times, each having a different target. This procedure allowed deduction of two separate optimum conditions, intended for the analysis of quality control and plasma samples, within the experimental domain. The predicted optimum for the quality control samples was: methanol-acetonitrile-15 mM K(2)HPO(4) buffer (pH 5.33) (10:42.08:47.92, v/v/v) as the mobile phase and 1.12 mL/min as the flow rate. The method using this optimized condition showed higher sensitivity and shorter analysis time than the previously published reports. The optimized assay condition was validated according to International Conference on Harmonization guidelines.

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Separation and estimation of seven vasodilators using packed column supercritical fluid chromatography

Cited by 16 publications

References 17 publications

Degradation studies of pentoxifylline: Isolation and characterization of a novel gem-dihydroperoxide derivative as major oxidative degradation product

Degradation studies of pentoxifylline: Isolation and characterization of a novel gem-dihydroperoxide derivative as major oxidative degradation product

Supercritical Fluid Chromatography: A Green Approach for Separation and Purification of Organic and Inorganic Analytes

An improved HPLC method with the aid of a chemometric protocol: Simultaneous analysis of amlodipine and atorvastatin in pharmaceutical formulations

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