Separation and evaluation of free radical‐scavenging activity of phenol components of green, brown, and black leaves of Bergenia crassifolia by using HPTLC‐DPPH^• method

Abstract

A new procedure has been developed to separate and quantify the free radical-scavenging activity of individual compounds from green, brown, and black leaves of Bergenia crassifolia based on the combination of high performance TLC (HPTLC) with a diode array detector (DAD) and postchromatographic 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*)) derivatization. Free gallic and ellagic acids, arbutin, hydroquinone, and bergenin in the B. crassifolia leaves' extracts were separated by HPTLC and identified. All compounds of the extract excluding bergenin were capable of scavenging DPPH * radicals. From the estimated ID(50) values, it can be seen that the increasing order of activity was gallic acid > arbutin > ellagic acid > hydroquinone > ascorbic acid. The antiradical activity of leaves of B. crassifolia is probably associated to the presence of phenol.

Adaptogenic effect of black and fermented leaves of Bergenia crassifolia L. in mice

“…crassifolia leaves are known to contain phenolic acids, phenols, tannins, flavonoids and bergenin and their content varies depending on the type of leaves analyzed Pozharitskaya et al, 2007). B. crassifolia L. leaves have been shown to possess antidiarrheal (Vereschagin et al, 1959), anti-inflammatory (Churin et al, 2005, antimicrobial (Kokoska et al, 2002), antioxidant , diuretic (Briukhanov and Fedoseeva, 1993) and immunostimulating (Popov et al, 2005) properties.…”

Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS

“…However, these methods might not be adopted widely because the online instrumental system required was complex and not commercial. A simple and feasible method, i.e., TLC separation combined with the detection of antioxidants in situ by postchromatographic derivatization, was also introduced [5,6]. Unfortunately, the separation ability of TLC was much lower than that of conventional HPLC so that several compounds might be co-eluted as one spot in TLC.…”

Diversity, pharmacology and synthesis of bergenin and its derivatives: Potential materials for therapeutic usages

“…Bergenin (1) itself is a good scavenger of hydroxyl radicals but not so effective in scavenging other free radicals like superoxide radical and DPPH [33,35,41,42,50,52,55,64,110,111,148,188,189]. Significant antioxidant activity of bergenin in hydrogen peroxide, ABTS, DPPH and inhibition of lipid peroxidation assays with IC 50 values 32.54, 75.06, 165.35 and 365.12 μg/mL, respectively, was reported by Srinivasan et al [54].…”

“…On NS3 serine protease activity assay, bergenin has displayed a weak activity against Hepatitis C virus (IC 50 = 1.71 mM) [137]. Antiviral activity of bergenin against herpes simplex virus type-1 showed an IC 50 value of (35) Astilbe rivularis Rhizome 0.0010 [27] Percentage of isolated yield is recorded in dried plant material weight basis from the available data.…”

“…TLC method was used for the quantification of bergenin in different parts of different Bergenia species [194][195][196][197]35] and Mallotus phillippensis [198]. Several researchers have used HPLC method to determine bergenin in genus Bergenia [39,197,[199][200][201][202], E. uchi [73], P. pterocarpum [203], Ardisia japonica [204] and Ficus racemosa [205,206].…”