Separation and functional characterization of human neutrophil subpopulations

Klempner, MS; Gallin, J I

doi:10.1182/blood.v51.4.659.bloodjournal514659

Cited by 14 publications

(19 citation statements)

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“…and it has been suggested that this increased activity is due to exposure to chemotactic factors in vivo (Kay et al, 19 79: Van Epps & Garcia, 1980). Secondly it has been shown that circulating neutrophils can be divided into various subpopulations when assessed by physical or functional parameters (Berkow & Bachner, 1985;Gallin, 1984), and that exudate neutrophils are composed of the more active populations (Klempner & Gallin, 1978). Thus in CAPD peritonitis more active subpopulations of circulating neutrophils respond to chemotactic signals and migrate into the peritoneum.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil function in patients on continuous ambulatory peritoneal dialysis

Harvey¹,

Sheppard²,

Morgan

et al. 1988

Br J Haematol

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Frequent and recurrent episodes of peritonitis are a major cause of morbidity in patients on continuous ambulatory peritoneal dialysis (CAPD). One factor contributing to this problem may be an abnormality of neutrophil function in these patients. We have therefore quantified phagocytosis and killing by circulating and peritoneal neutrophils from patients on CAPD with and without peritonitis. Circulating neutrophils from uninfected patients showed reduced phagocytosis of both Staphylococcus epidermidis and Candida guilliermondii because of an opsonic defect in CAPD serum and because of a defect of the neutrophils themselves. In contrast, phagocytosis by circulating and peritoneal neutrophils from patients with peritonitis was normal. Intracellular killing of C. guilliermondii was normal in all groups of neutrophils but killing of S. epidermidis, the organism most commonly isolated in CAPD peritonitis, was reduced. The possible mechanisms for the enhanced neutrophil activity seen in peritonitis, and for the decreased killing of S. epidermidis in contrast to normal killing of C. guilliermondii are discussed. A defect in killing of S. epidermidis may explain why peritonitis caused by this organism can be difficult to erradicate.

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Section: Discussionmentioning

confidence: 99%

Neutrophil function in patients on continuous ambulatory peritoneal dialysis

Harvey¹,

Sheppard²,

Morgan

et al. 1988

Br J Haematol

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“…Residual erythrocytes were lysed with two cycles of hypotonic saline. Cells were then washed in PBS solution and finally suspended in PBS added with CaCl 2 (100 mM), MgCl 2 (50 mM), and glucose (100 mg/mL) (Klempner and Gallin, 1978). Cell viability was obtained by counting on a Neubauer chamber and verified by Trypan Blue (0.6%) exclusion; viability was considered acceptable above 95%.…”

Section: Neutrophil Isolationmentioning

confidence: 99%

Basal neutrophil function in human aging: Implications in endothelial cell adhesion

Nogueira‐Neto

Cardoso

Monteiro

et al. 2016

Cell Biology International

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Much attention has been drawn to the pro-inflammatory condition that accompanies aging. This study compared parameters from non-stimulated neutrophils, obtained from young (18-30 years old [y.o.]) and elderly (65-80 y.o.) human volunteers. Measured as an inflammatory marker, plasmatic concentration of hs-CRP was found higher in elderly individuals. Non-stimulated neutrophil production of ROS and NO was, respectively, 38 and 29% higher for the aged group. From the adhesion molecules evaluated, only CD11b expression was elevated in neutrophils from the aged group, whereas no differences were found for CD11a, CD18, or CD62. A 69% higher non-stimulated in vitro neutrophil/endothelial cell adhesion was observed for neutrophils isolated from elderly donors. Our results suggest that with aging, neutrophils may be constitutively producing more reactive species in closer proximity to endothelial cells of vessel walls, which may both contribute to vascular damage and reflect a neutrophil intracellular disrupted redox balance, altering neutrophil function in aging.

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“…For example, Sabin observed in 1928 that some neutrophils appear to have better function and motility than others, and recently two populations of circulating neutrophils have been described, one which forms rosettes with IgG-coated erythrocytes and the other which does not. 4 The rosetteforming cells appear to comprise approximately 80% of the circulating neutrophil population and have normal chemotaxis, phagocytosis, and killing. The remaining 20% of non-rosette-forming neutrophils have less adequate function, raising the possibility that variations in the distribution of such cell populations may modify the risk of infection in a neutropenic patient.…”

Section: Granulopoiesis and Kineticsmentioning

confidence: 99%

Granulocytopenia and cancer therapy: Past problems, current solutions, future challenges

Pizzo

1984

Cancer

121

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Granulocytopenia has been closely associated with cancer and its treatment. The risk for a life‐threatening infection when the granulocyte count falls below 500/mm3 not only is an important complication of therapy and a major cause of death in cancer patients but also plays an important role in the design, schedule, and doses of cancer treatment regimens. While granulocytopenia remains an unavoidable complication of current therapy, improved recognition of patients at risk and prompt initiation of aggressive supportive care have had a significant impact on reducing its infection‐related morbidity and mortality. In particular, early empiric antibiotic therapy when the granulocytopenic patient becomes febrile has resulted in a significant reduction in the early mortality from undiagnosed bacterial infections. New antibiotics (extended‐spectrum penicillins, third generation cephaloporins) provide new options because of their broad efficacy and potential for reducing the toxicity of antimicrobial therapy. However, as bacterial infections have become better controlled, fungi have emerged as important pathogens. Early aggressive empiric antifungal therapy appears to have reduced infectious mortality, although the repertoire of effective antifungal agents is quite limited. Considerable efforts have been expended in trying to replenish graulocytes by transfusion in infected patients, but technical deficiencies have limited this approach. Current and future efforts are directed toward refining management as well as to developing methods to improve host defenses and provide prophylaxis against infections. If the full potential of current cancer therapy is to be realized, control of granulocytopenia and the prevention of infections are essential goals.

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Separation and functional characterization of human neutrophil subpopulations

Cited by 14 publications

References 0 publications

Neutrophil function in patients on continuous ambulatory peritoneal dialysis

Neutrophil function in patients on continuous ambulatory peritoneal dialysis

Basal neutrophil function in human aging: Implications in endothelial cell adhesion

Granulocytopenia and cancer therapy: Past problems, current solutions, future challenges

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