Separation and identification of ACE inhibitory peptides from defatted walnut meal

Chen, Yonghao; Li, Jun; Dong, Ningguang; Lu, Xiaodan; Hao, Yulan; Qi, Jianxun

doi:10.1007/s00217-020-03553-5

Cited by 16 publications

(13 citation statements)

References 39 publications

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“…Chen et al. (2020) revealed that the peptide obtained from the protein enzymatic hydrolysate of a walnut meal had high ACE inhibitory activity, and the key peptides were GVVPHN, EHSLDPLK, and KTLLNFGPN. Xu et al.…”

Section: Introductionmentioning

confidence: 99%

“…indicated that walnut oligopeptides have protective effects against ethanolinduced gastric mucosal injury in vivo through antiinflammatory, antioxidation, and antiapoptosis mechanisms. Chen et al (2020) revealed that the peptide obtained from the protein enzymatic hydrolysate of a walnut meal had high ACE inhibitory activity, and the key peptides were GVVPHN, EHSLDPLK, and KTLLNFGPN. Xu et al (2019) studied the walnut protein hydrolysate could regulate the components and structural degradation of photoaged skin in mice.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Tian

Sun

et al. 2021

Journal of Food Science

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Defatted walnut kernel with pellicle (WKP) is an industrial byproduct during walnut oil extraction, which is rich in protein and polyphenols. WKP was hydrolyzed by simulated gastrointestinal digestion to obtain WKP hydrolysates (WKPHs). Results showed the protein recovery and hydrolysis degree of WKPH were 82.15 and 10.36%. The total phenol contents in WKP and WKPH were 4.90 and 40.70 mg gallic acid equivalent/g, respectively. The antiaging activity of WKPH was evaluated using a d‐gal‐induced aging mouse model. Results showed that WKPHs could recover the activities of SOD and T‐AOC and the content of MDA in tissues and serum of the aging mice. The histological morphology of liver and kidney sections and the immunohistochemistry of TNF‐α, IL‐1β, and IL‐6 in liver were observed. WKPH could effectively protect the tissue structure of the liver and kidney and reduce the inflammatory expression of liver in aging mice. The polypeptides and polyphenols in WKPH were further analyzed. Fifty polypeptides were identified and 12 of these peptides had Leu‐Arg at the C‐terminal. Forty‐two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study provided a theoretical basis for the improved processing and high‐value utilization of walnut byproducts. Practical Application Defatted walnut kernel with pellicle was hydrolyzed by simulated gastrointestinal digestion to obtain its hydrolysates. The hydrolysates have good antiaging activity in vivo. Fifty polypeptides were identified and 12 of these peptides had Leu‐Arg at the C‐terminal. Forty‐two polyphenols were detected, and most phenolic compounds belonged to ellagitannins. This study could provide a theoretical basis for high‐value utilization of walnut byproducts.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Tian

Sun

et al. 2021

Journal of Food Science

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“…However, its use with yogurt starter cultures had higher effectiveness than the control sample. We believe that this was due to the high ACE-I activity of yogurt starter strains, as indicated in some previous studies (Zhang et al 2020;Chen et al 2020), and to the increase in bioactive peptides, which resulted in the interaction of these yogurt cultures and co-cultures.…”

Section: The Ace-i Inhibitory Activitiesmentioning

confidence: 56%

Biotechnological uses of functional Lacticaseibacillus paracasei subsp. tolerans NOC-122, Levilactobacillus parabrevis NOC-111, and Latilactobacillus curvatus NOC-110

Özdemir

2022

Preprint

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This study aimed to reveal the physicochemical and organoleptic effects of three functional LAB isolates in a milk medium: Lacticaseibacillus paracasei subsp. tolerans NOC-122, Levilactobacillus parabrevis NOC-111, and Latilactobacillus curvatus NOC-110. Firstly, a total of 200 indigenous LAB strains isolated from artisanal tulum cheeses were screened for potential proteolytic activity, lipolytic activity, citrate-lyase-synthesizing ability, and EPS-producing ability. Furthermore, a total of six fermentates were produced using these strains as a single culture or as a co-culture with yogurt bacteria. The physicochemical and microbiological properties, the activity of angiotensin-converting enzyme (ACE) inhibitors, and the amino acid and volatile aroma compound profiles of them were determined. In addition, the changes in all these properties during the storage process were determined. According to the results, NOC-110 and NOC-122 were effective in increasing the ACE inhibitory activity. On the other hand, NOC-122 was responsible for a fresh cheesy, slightly oily flavor when used as a single culture. As for the others, NOC-111 gave a fresh, fruity, and slightly herbal flavor; NOC-110 gave a flavor similar to that of NOC-122 when they were used as a single culture. Also, co-cultures of the strains were investigated. The results of the study are a guide for the usability of these isolates as single cultures or co-cultures in the production of dairy-based food. These findings can shed light on many future studies and innovative food products.

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“…The IC 50 values of the DPP‐IV inhibitory activity of LNFDPNR and LPQQQAQFK were 471.47 ± 5.19 and 489.88 ± 1.63 μmol L −1 . The DPP‐IV inhibitory activity of the two peptides was stronger than that of LGRNLPPI, 46 SEPA, 47 and GVVPHN 48 and weaker than that of LPVPQ 49 and ELHQEEPL 50 . On the basis of activity verification, the two peptides obtained through the screening methods had good ACE and DPP‐IV inhibitory activities.…”

Section: Resultsmentioning

confidence: 93%

Identification of in vitro angiotensin‐converting enzyme and dipeptidyl peptidase IV inhibitory peptides from draft beer by virtual screening and molecular docking

Tian

Hu²,

Zhuang

et al. 2021

J Sci Food Agric

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BACKGROUND: Hypertension and diabetes are two kinds of senile diseases which often occur simultaneously. The commonly used drugs in clinic may produce certain side effects. Food-derived polypeptide is a kind of polypeptide with great development potential, which has many functions of regulating human physiological function. Beer is rich in nutrition but there are few researches on bioactive peptides in beer.RESULTS: In this study, a rapid virtual screening method was established to obtain bioactive peptides from Tsingtao draft beer. The peptide sequence was analyzed by ultra-performance liquid chromatography-quadrupole-Orbitrap-tandem mass spectrometry (UPLC-Q-Orbitrap-MS 2 ), and 50 peptides were identified. Eight peptides with potential biological activities were screened by using Peptide Ranker software and previous literature references. On the basis of absorption prediction, toxicity prediction, and molecular docking analysis, LNFDPNR and LPQQQAQFK were finally confirmed. The molecular docking results showed that two peptides could bind angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) tightly by hydrogen bonding and hydrophobic interaction. The in vitro activity evaluation results showed that two peptides had obvious ACE and DPP-IV inhibitory activity.CONCLUSION: This study established a method for rapidly screening bioactive peptides from Tsingtao draft beer, screened two ACE and DPP-IV inhibitory peptides in beer and analyzed their active action mechanism. This article may have great theoretical significance and practical value to further explore the health function of beer.

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Separation and identification of ACE inhibitory peptides from defatted walnut meal

Cited by 16 publications

References 39 publications

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Protective effect againstd‐gal‐induced aging mice and components of polypeptides and polyphenols in defatted walnut kernel during simulated gastrointestinal digestion

Biotechnological uses of functional Lacticaseibacillus paracasei subsp. tolerans NOC-122, Levilactobacillus parabrevis NOC-111, and Latilactobacillus curvatus NOC-110

Identification of in vitro angiotensin‐converting enzyme and dipeptidyl peptidase IV inhibitory peptides from draft beer by virtual screening and molecular docking

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