Separation and identification of cis/trans carotenoid isomers

Saleh, Mohammed H.; Tan, Barrie

doi:10.1021/jf00008a015

Cited by 54 publications

(40 citation statements)

References 15 publications

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“…The separation of carotenoids and their cb isomers are routinely conducted by HPLC using a Cis column [6,7,14]. However, the HPLC conditions used by these authors failed to resolve all-trans and cis forms of lycopene.…”

Section: Resultsmentioning

confidence: 99%

Separation of lycopene and itscis isomers by liquid chromatography

Lee

Chen

2001

Chromatographia

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SummaryLycopene is an important biological compound that is widely distributed in fruits and vegetables. Epidemiological study has shown that the dietary intake of lycopene may reduce the risk of certain types of cancers such as prostate cancer. However, the formation of c/s-isomers of lycopene during food processing and storage may decrease its biological activity Thus, it is important to learn about the content of lycopene and its cis isomers in foods. In this studywe compared I',,vo types of columns (C18 and C30) and various solvent systems for the separation of lycopene and its cis isomers by HPLC. Results showed that all-trans-lycopene and its nine cis isomers could be resolved by employing a C30 column with a mobile phase of n-butanol-acetonitrile-methylene chloride (30:70:10, v/v/v) and detection at 476 nm within 35 min. A C30 column was found to provide more powerful resolution of lycopene and its cis isomers, but the retention time was drastically increased compared to that of a C18 column. The c/s-isomers of lycopene were tentatively identified as 5-cis, 9-cis, 13-cis, 15-cis-lycopene, and possibly as four lycopene di-cis isomers, based on spectral characteristics and Q ratios as reported in the literature. The method developed in this study could be applied to determine the lycopene content in tomatoes.

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Section: Resultsmentioning

confidence: 99%

Separation of lycopene and itscis isomers by liquid chromatography

Lee

Chen

2001

Chromatographia

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“…C 30 columns provide better resolutions between carotenoids with similar polarity compared to C 18 columns, so they are normally the columns of choice for the separation of geometrical isomers (Lacker et al, 1999;Sander et al, 1999Sander et al, , 2000. Although on some occasions C 18 stationary phases, mainly Vydac C 18 columns, have yielded relatively good separations of these isomers (Saleh and Tan, 1991;Chen et al, 1996;Corte´s et al, 2004), their efficiency not only in the separation of geometrical isomers, but also of carotenoids with similar polarity, is clearly lower in comparison to C 30 columns, as it was clearly concluded early in the development of the latter stationary phase (Sander et al, 1994).…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

Review: Analysis of carotenoids in orange juice

Meléndez-Martínez

Vicario

Heredia

2007

Journal of Food Composition and Analysis

131

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“…18 The analytical apparatus was the same as that used for phenolic separation. Carotenoids were separated along a Vydac column 201 TP 54 C18, 5 mm (250 mm Â 4.6 mm id) and a guard column (15 mm Â 3.2 mm id, Vydac) with the isocratic mobile phase MeOH/acetonitrile/dichloromethane 18:80:2.…”

Section: Separation and Quantification Of Carotenoids And Tocopherolsmentioning

confidence: 99%