Separation and identification of phytosiderophores and their metal complexes in plants by zwitterionic hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry

Xuan, Yue; Scheuermann, Enrico B.; Meda, Anderson Rotter; Hayen, Heiko; Wirén, Nicolaus von; Weber, G.

doi:10.1016/j.chroma.2006.09.060

Cited by 72 publications

(81 citation statements)

References 38 publications

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“…The same elution order of free ligands was already observed in HPLC at pH 7.3, using a special zwitterionic column operated in hydrophilic interaction LC (HILIC) mode [27]. Interestingly, that separation is also based on a combination of hydrophilic interactions (i.e., partitioning of analytes from a more hydrophobic eluent into the aqueous layer at a hydrophilic stationary phase) and electrostatic interactions.…”

Section: Ce Separation Of Ps and Namentioning

confidence: 65%

“…Comparing the above results with our chromatographic data [27], it must be noted that the CE separation of several divalent metal species is not as good as in LC, but on the other hand, Fe(III) species are separated without dissociation or other problems, and this is a clear advantage of CE.…”

Section: Ce Separation Of Metal Speciesmentioning

confidence: 79%

“…However, the biggest problem in nearly all published analytical methods for trace metal speciation is not the detection, but the unknown stability of metal species with respect to the (liquid chromatographic) separation. Such problems were reported for Ni species [23], Fe species [22], and even for PS species that are thermodynamically very stable, partial dissociation may happen on chromatographic columns [26,27]. One solution to this dilemma could be the use of direct methods for metal speciation, i.e., methods that are not based on a preceding separation.…”

Section: Introductionmentioning

confidence: 93%

“…7, the separation of the two Fe(III)-EDDHA forms is shown on a ZIC-CE capillary with increasing amounts of ACN in the BGE. The ZIC-CE capillary is the CE version of the ZIC-HILIC column, which has been used in HPLC for separation of PS and respective metal species [27]. It consists of a silica capillary with covalently attached sulfobetaine groups.…”

Section: Ce Separation Of Eddha and Fe(iii)-eddha Isomersmentioning

confidence: 99%

“…Both methods are applicable for quantitative analyses of PS in the low micromolar concentration range, but are not well suited for the detection of intact metal species. In the last few years, some hyphenated analytical methods for detection and quantitative determination of metal-PS species have been proposed, based on HPLC with atomic absorption spectrometric detection [22], HPLC with inductively coupled plasma mass spectrometric detection (ICP/MS) [23][24][25][26], or HPLC-ESI/MS [24,25,27]. Clearly, the ICP/MS methods offer very good sensitivity and element specificity, but lack unequivocal identification of unknown species.…”

Section: Introductionmentioning

confidence: 99%

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CE of phytosiderophores and related metal species in plants

et al. 2007

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Phytosiderophores (PS) and the closely related substance nicotianamine (NA) are key substances in metal uptake into graminaceous plants. Here, the CE separation of these substances and related metal species is demonstrated. In particular, the three PS 2'-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), and NA, are separated using MES/Tris buffer at pH 7.3. Moreover, three Fe(III) species of the different PS are separated without any stability problems, which are often present in chromatographic analyses. Also divalent metal species of Cu, Ni, and Zn with the ligands DMA and NA are separated with the same method. By using a special, zwitterionic CE capillary, even the separation of two isomeric Fe(III) chelates with the ligand ethylenediamine-N,N'-bis(o-hydroxyphenyl)acetic acid (EDDHA) is possible (i.e., meso-Fe(III)-EDDHA and rac-Fe(III)-EDDHA), and for fast separations of NA and respective divalent and trivalent metal species, a polymer CE microchip with suppressed EOF is described. The proposed CE method is applicable to real plant samples, and enables to detect changes of metal species (Cu-DMA, Ni-NA), which are directly correlated to biological processes.

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Section: Ce Separation Of Ps and Namentioning

confidence: 65%

Section: Ce Separation Of Metal Speciesmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 93%

Section: Ce Separation Of Eddha and Fe(iii)-eddha Isomersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CE of phytosiderophores and related metal species in plants

et al. 2007

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Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix‐assisted laser desorption/ionization‐based proteomic analysis

Intoh

Kurisaki

Fukuda

et al. 2009

Biomedical Chromatography

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Complexation of metals by phytosiderophores revealed by CE‐ESI‐MS and CE‐ICP‐MS

et al. 2010

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CE-ESI-MS and CE-ICP-MS were implemented for studying three phytosiderophores (mugineic acid, epi-mugineic acid and deoxymugineic acid) and their metal complexes. Free ligands and ferric complexes were analyzed using the first methodology, while six free metals (Co(II), Cu(II), Fe(III), Mn(II), Ni(II) and Zn(II)) together with the corresponding complexes were investigated by the latter technique. CE separation was realized at a voltage of +25 kV employing a BGE containing 20 mM ammonium bicarbonate at pH 7.2. Both techniques revealed limits of detection in the high nM to low microM range. Standard additions to hydroponic samples of H. distichon, cv. Bodega (spring barley) showed regression coefficients for the metal-ligand complexes ranging from 0.984 to 0.999. Additionally, results of a competitivity study allowed the determination of relative metal-phytosiderophore complex stability constants of deoxymugineic/mugineic acid.

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Separation and identification of phytosiderophores and their metal complexes in plants by zwitterionic hydrophilic interaction liquid chromatography coupled to electrospray ionization mass spectrometry

Cited by 72 publications

References 38 publications

CE of phytosiderophores and related metal species in plants

CE of phytosiderophores and related metal species in plants

Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix‐assisted laser desorption/ionization‐based proteomic analysis

Complexation of metals by phytosiderophores revealed by CE‐ESI‐MS and CE‐ICP‐MS

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