Separation and Purification of Glucose in Sake for Carbon Stable Isotope Analysis

Carbon stable isotopes of glucose during the degradation of rice by the koji fungus Aspergillus oryzae

“…Glucose was separated from the samples following Akamatsu et al [ 29 ], using cation-exchange (1 mL Dionex OnGurad II H) and anion-exchange (1 mL Dionex OnGurad II A, Thermo Fisher Scientific, Waltham, MA, USA) solid-phase extraction cartridges to remove amino acids and organic acids to prevent contamination of the columns during HPLC purification. The cartridges were connected in this order, and a 5 mL syringe was attached on top of the cation-exchange cartridge.…”

Quartz Crystal Microbalance as a Holistic Detector for Quantifying Complex Organic Matrices during Liquid Chromatography: 2. Compound-Specific Isotope Analysis

“…In contrast, only a factor of approximately 20 can be gained for an early-eluting compound. These findings are meaningful since the challenge of separating small polar compounds using RP columns is well known. − Potentially, a column phase engineered for these compounds (e.g., HILIC) , could result in a better separation of early-eluting compounds and NOM and thus a higher NOM removal during purification. To put these findings in a larger context of complete sample preparation for carbon CSIA, an overall higher removal can become possible when combining the targeted HPLC cleanup presented here with the use of more selective SPE materials (e.g., cyclodextrins) to replace Oasis HLB in the first extraction step, making it possible to measure concentrations ≥100 ng/L for BAM and ≥3 ng/L for BOSC in a groundwater sample containing 0.5 mgC/L NOM.…”