Separation and purification of magnoflorine, spinosin, and 6‴‐feruloyspinosin from Ziziphi Spinosae Semen by high‐speed counter‐current chromatography

Abstract

In the study, high-speed counter-current chromatography was used for separation and purification of magnoflorine, spinosin, and 6‴-feruloyspinosin from Ziziphi Spinosae Semen. With n-butanol-ethyl acetate-water (2:3:5, v/v) as the optimum solvent system, about 75 mg of magnoflorine, 110 mg of spinosin, and 40 mg of 6‴-feruloyspinosin were isolated from 0.5 g of crude extract of Z. Spinosae Semen, with the purity of 95.7, 97.2, and 96.4%, respectively. The chemical structures were identified by MS and NMR spectroscopy. In addition, the antidepressant activity of the isolated components was evaluated by PC12 cells injury model and chronic unpredictable mild stress depression mouse model. The results showed that high-speed counter-current chromatography could be used to realize the one-time rapid preparation and separation of magnoflorine, spinosin, and 6‴-feruloyspinosin from Z. Spinosae Semen and compatibility of these isolated components has certain antidepressant activity.

Exploration of Ziziphi Spinosae Semen in Treating Insomnia Based on Network Pharmacology Strategy

“…Jujuboside A can inhibit cancer cell growth through various mechanisms such as cell cycle arrest, proliferation inhibition, stem cell inhibition, and promotion of aging and can contaminate antitoxins [ 25 , 26 ], and our analysis results show that there are many intersections of targets associated with it and insomnia targets. Spinosin exerts neuroprotective effects by inhibiting oxidative damage and has a significant antidepressant effect, and there is a great association between spinosin and insomnia [ 27 , 28 ]. Although the kinetic values of these two components are relatively low, they have very excellent biological activity and are closely related to our study.…”

Recent trends in multidimensional countercurrent chromatography

“…The isolation and refinement of 6‴-feruloyspinosin, spinosin, and magnoflorine from Ziziphi Spinosae Semen were accomplished through a combination of A TBE-1000A HSCCC and HPLC under the Agilent C-18column (4.6 × 250 mm, 5 μm). The isolated constituents were distinguished via the implementation of MS, 1 H-NMR, and 13 C-NMR spectroscopic analyses [64]. In addition, compared to its one-dimensional counterpart, prep-HPCCCbased multidimensional chromatography stands out with its exceptional ability to isolate and purify.…”

Ziziphi Spinosae Semen : An updated review on pharmacological activity, quality control, and application

“…Also, the polysaccharide (yield was 1.05 ± 0.08%) was extracted from ZSS with ultrasound-assisted (temperature was 52.5 °C, time was 21.2 min, power was 134.9 W) (Lin et al, 2018). In 2021, Xu et al (2021) separated magnoflorine (75 mg), spinosin (110 mg), and 6‴-feruloylspinosin (40 mg) from ZSS (0.5 g) by high-speed countercurrent chromatography, with a purity of 95.7%, 97.2%, and 96.4%, respectively. In addition, the yields of vitamin E and squalene obtained by the optimized ultrasonic-assisted extraction process were 29.58 ± 0.19 mg/100 g and 820.49 ± 3.66 μg/100 g, respectively (Xiao et al, 2018).…”