Separation and Purification of Soluble Polymers and Cell Wall Fractions from Wheat, Rye and Hull less Barley Endosperm Flours for Structure-Nutrition Studies

Abstract

The nutritional values associated with the cell walls of cereal endosperm flours are due to a combination of solubilized arabinoxylan and (1-3,1-4)-β-d-glucan as well as residual nonsolubilized cell wall material. In order to investigate structure-nutrition relationships, an appropriate method for the complete functional and structural characterization of cell wall polysaccharides in various cereal endosperm flours is described. This involves the separation of soluble polymers and the residual cell wall fraction without using organic solvents, and the fractionation of soluble polymers into arabinoxylan- and (1-3,1-4)-β-d-glucan-rich fractions for subsequent analysis. This methodology is applied to endosperm flours from wheat, hull-less barley and rye, and could be extended to include studies on the effects of food processing with respect to yield and characteristics of the three fractions in order to better understand the structural basis for nutritional functionality.

Soluble Dietary Fiber Reduces Trimethylamine Metabolism via Gut Microbiota and Co‐Regulates Host AMPK Pathways

“…Inoculation with Rhizopus oryzae and exposure to steam were used for producing fermented and steam‐exploded wheat bran, respectively. SDFs were extracted from wheat brans and further treated by α‐amylase and protease E (7892 and 1020 U mL –1 ; Novozymes Biotechnology Co., Ltd., Tianjin, China), and amyloglucosidase (1,176 U g –1 , Tianjin Noao Science & Technology Development Co., Ltd., Tianjin, China) for removing starch and protein according to a previous method . The saccharide composition of the SDF fractions and their molecular weight and structural features were determined using colorimetric analysis, high performance liquid chromatography (HPLC), and gas chromatography (GC) profiling as described previously and the results are presented in Table 1, Supporting Information.…”

A Genome Wide Association Study of arabinoxylan content in 2-row spring barley grain

“…Monosaccharide analysis was carried out essentially as described by Comino et al [97] with some modifications. Samples were treated with 1 mL 1 M sulphuric acid at 100°C for 3 hours.…”

Grape marc as a source of carbohydrates for bioethanol: Chemical composition, pre-treatment and saccharification

“…Briefly, the material (30 mg) was incubated with 13.5 M H 2 SO 4 at room temperature for 1 h, followed by dilution to 0.75 M H 2 SO 4 and autoclaving at 121°C (Tuttnauer 3850 ELC Benchtop Sterilizer). The solubilized material and the residual biomass were separated by centrifugation for 10 min at 10 000 g. The supernatant was analyzed for monosaccharides (Comino et al, 2013). A sugar recovery standard for monosaccharides was carried through the acid hydrolysis to compensate for degradation of monomers during the hydrolysis step.…”

“…Following polysaccharide isolation, the fractions were dialyzed and lyophilized (monosaccharides were quantified by HPLC(Fig. 1)(Comino et al, 2013).…”