Separation methods for alkylating antineoplastic compounds

Paci, Angélo; Rieutord, André; Brion, F.; Prognon, P.

doi:10.1016/s0378-4347(01)00280-8

Cited by 16 publications

(11 citation statements)

References 151 publications

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“…Mean extraction recoveries were 60%, 55%, 95% and 101% for N 2 -DCE-IFM, N 3 -DCE-IFM, IFM and CPM, respectively (see Table 4). These values agreed with those previously described with ethylacetate extraction for the metabolites and IFM [9].…”

Section: Evaluation Of the Matrix Effect And The Recoverysupporting

confidence: 92%

“…The direct quantitative determination of the oxazaphosphorines, such as IFM and cyclophosphamide (CPM), and their metabolites is difficult, because of their high polarity and their chemical and thermal properties. Thus, several analytical methods have been developed using gas chromatography (GC) or high performance liquid chromatography (HPLC) combined with different detection techniques [9]. The UV detection of oxazaphosphorine compounds is also problematic due to their poor spectral properties.…”

Section: Introductionmentioning

confidence: 99%

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Liquid chromatography–mass spectrometry assay for quantitation of ifosfamide and its -deschloroethylated metabolites in rat microsomal medium

Storme¹,

Mercier²,

Deroussent³

et al. 2005

Journal of Chromatography B

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Section: Evaluation Of the Matrix Effect And The Recoverysupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–mass spectrometry assay for quantitation of ifosfamide and its -deschloroethylated metabolites in rat microsomal medium

Storme¹,

Mercier²,

Deroussent³

et al. 2005

Journal of Chromatography B

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“…[11][12][13][14]. Briefly, 30 min after injection of cyclophosphamide, mice were sacrificed and tumors carefully excised.…”

Section: Extraction Of Hydroxycyclophosphamide From Tumor and Blood Smentioning

confidence: 99%

Potentiation of cyclophosphamide chemotherapy using the anti-angiogenic drug thalidomide: Importance of optimal scheduling to exploit the ‘normalization’ window of the tumor vasculature

et al. 2006

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The aim of this work was to study how administration schedule affects potentiation of cyclophosphamide, an alkylating agent, by thalidomide, an anti-angiogenic agent. Tumor oxygenation after thalidomide administration was determined over time by EPR oximetry. Such measurements provide a surrogate marker for determining the timing of 'normalization' of tumor vasculature. Re-growth delays were measured using different combinations and schedules of treatments. Additionally, the uptake of the metabolite of cyclophosphamide (hydroxycyclophosphamide or OH-CP) into tumors was determined by high performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). A significant increase in pO 2 was observed after 2 and 3 days of treatment before eventually declining on day 4. Thalidomide potentiated the effect of cyclophosphamide only when cyclophosphamide was administered after 2 days of treatment with thalidomide (no significant benefit using other schedules). In this time frame, the HPLC/MS/MS measurements showed that the quantity of OH-CP penetrating into the tumor was about twice in mice treated by thalidomide compared to controls. In conclusion, the present study demonstrates that the benefit of combined therapy using an anti-angiogenic agent with a cytotoxic agent requires knowledge of the time window during which the vessels initially become normalized. q

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“…Od predstavnika alkil-sulfonata najvažniji je busulfan, bifunkcionalni agens koji specifi čno reagira sa -SH-skupinama u aminokiselinama i proteinima (17). U podskupinu nitrozourea ubrajaju se karmustin ili biskloretil nitrozourea (BCNU) koji djeluje bifunkcionalno te lomustin ili cikloheksil-kloretilnitrozourea (CCNU) i semustin ili metil-CCNU, kao monofunkcionalna alkilirajuća sredstva (19). U ostale alkilirajuće lijekove ubraja se dakarbazin (17).…”

Section: Alkilirajući Lijekoviunclassified

Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

Kopjar

Želježić

Kašuba

et al. 2010

Archives of Industrial Hygiene and Toxicology

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Nevenka KOPJAR, Davor ŽELJEŽIĆ, Vilena KAŠUBA i Ružica ROZGAJ Institut za medicinska istraživanja i medicinu rada, Zagreb, HrvatskaPrimljeno u siječnju 2010. Prihvaćeno u veljači 2010.U članku je prikazana osnovna podjela antineoplastičnih lijekova prema mehanizmima djelovanja na razini stanice. Objašnjeni su mehanizmi genotoksičnosti najvažnijih vrsta lijekova koji se primjenjuju u okviru uobičajenih protokola za liječenje zloćudnih novotvorina. Navedena je važeća klasifi kacija antineoplastika prema kancerogenom potencijalu, podaci o mutagenom potencijalu te je prikazana njihova podjela u skladu s anatomsko-terapijsko-kemijskim sustavom klasifi kacije. Sustavno su prikazani najvažniji rezultati svjetskih i hrvatskih istraživanja na populacijama radnika izloženih antineoplasticima, provedenih u razdoblju 1980.-2009. s pomoću četiri najčešće primjenjivane metode: analize izmjena sestrinskih kromatida, analize kromosomskih aberacija, mikronukleus-testa i komet-testa. Objašnjena su osnovna načela navedenih metoda te raspravljene njihove prednosti i nedostaci. Biološki pokazatelji daju važne podatke o individualnoj osjetljivosti profesionalno izloženih ispitanika koji mogu poslužiti unaprjeđenju postojećih uvjeta rada i upravljanju rizicima pri izloženosti genotoksičnim agensima. Na osnovi prednosti i nedostataka citogenetičkih metoda zaključeno je da je mikronukleus-test, koji podjednako uspješno dokazuje klastogene i aneugene učinke, jedna od najboljih metoda dostupnih za otkrivanje štetnih djelovanja antineoplastičnih lijekova koji su u aktivnoj primjeni.

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Separation methods for alkylating antineoplastic compounds

Cited by 16 publications

References 151 publications

Liquid chromatography–mass spectrometry assay for quantitation of ifosfamide and its -deschloroethylated metabolites in rat microsomal medium

Liquid chromatography–mass spectrometry assay for quantitation of ifosfamide and its -deschloroethylated metabolites in rat microsomal medium

Potentiation of cyclophosphamide chemotherapy using the anti-angiogenic drug thalidomide: Importance of optimal scheduling to exploit the ‘normalization’ window of the tumor vasculature

Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

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