Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography

Hancu, Gabriel; Gáspár, Attila; Gyéresi, Árpád

doi:10.1016/j.jbbm.2006.02.003

Cited by 45 publications

(22 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrolysis in acidic solution leads generally to 2-aminobenzophenone derivatives, through the splitting of the N1-C2 bond of the diazepine ring [29]. Bromazepam like most of the benzodiazepine derivatives is hydrolyzed in acidic aqueous solutions leading to a series of degradation products.…”

Section: Hydrolysis Of Bromazepam Under Acidic Conditionmentioning

confidence: 99%

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Takele

Attaya²,

Hymete³

et al. 2017

PHARMSCI

View full text Add to dashboard Cite

Introduction:Bromazepam is hydrolyzed in acidic aqueous solution leading to a series of degradation products. The rate of acidic hydrolysis is believed to be dependent on the state of protonation of the pyridyl and azomethine nitrogen atoms. Stability test is important in pharmaceutical industry to provide evidence on how the quality of an active substance or pharmaceutical product varies with time under the influence of a variety of environmental factors. Objective:The aim of the study was to develop a simple stability indicating method for the determination of bromazepam. Method:Bromazepam solution was prepared and forced degradation of bromazepam was performed under acid hydrolysis using sulphuric acid. High performance liquid chromatography determination of pure and degraded bromazepam and bromazepam-copper (II) complex was performed using reversed phase octyl C-8 column under isocratic conditions and the chromatographic conditions were set as follows; the flow rate of the mobile phase was 1.5 mL/min; injection volume was 10 μL, column temperature was 30 o C and the detector wavelength being 309 nm. Results:Bromazepam, its degradation product and bromazepam chelated with copper (II) were determined using the developed mobile phase with flow rate of 1.5 mL/min. Good separation with sharp peak, minimum tailing and retention time repeatability was obtained. The rate order, rate constant and half-life of degradation were also determined, and it was observed that the degradation reaction follows the first order kinetics. Conclusion:Chromatographic separation of bromazepam chelated with copper (II) was achieved and the method can be further used in drug manufacturing quality control.

show abstract

Section: Hydrolysis Of Bromazepam Under Acidic Conditionmentioning

confidence: 99%

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Takele

Attaya²,

Hymete³

et al. 2017

PHARMSCI

View full text Add to dashboard Cite

show abstract

“…It is interesting that peak splitting didn't occurred when we used β-CD as buffer additive, but separation of the two enantiomers can be solved using hydroxypropyl-β-CD (HP-β-CD) as additive, because of the different affinities displayed by the selector molecules towards the two enantiomers. 17 The analytical performance of the optimized method was evaluated on the basis of precision (by calculating regional standard deviation-RSD for the migration time and peak area), linearity and limit of detection (LOD).…”

Section: 10mentioning

confidence: 99%

“…3,7 The anionic SDS micelles are electrostatically attracted towards the anode, but because of the electroosmotic flow (EOF) which is usually stronger than the electrophoretic migration, the anionic micelles will slowly migrate towards the cathode, in the direction of the detector. 7,8 Our previous studies 9,10 showed that in order to improve the separation of BZD we have to use additives (organic solvents -methanol, acetonitrile) to modify the aqueous phase consequently manipulating selectivity. In general, addition of organic solvents reduces the electroosmotic velocity and, hence, expands the migration time window, improving selectivity of the separation.…”

Section: Introductionmentioning

confidence: 99%

“…In general, addition of organic solvents reduces the electroosmotic velocity and, hence, expands the migration time window, improving selectivity of the separation. 9,11,12 Another alternative would be the addition of cyclodextrines (CDs) to the buffer solution. CDs are cyclic oligosaccharides having an external hydrophilic surface and a hydrophobic cavity, in which they can include other compounds, which fit in their cavity by hydrophobic interaction.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Hancu¹,

Gyéresi²

2011

Croat. Chem. Acta

Self Cite

View full text Add to dashboard Cite

Abstract. The applicability of micellar electrokinetic capillary chromatography (MEKC) for the separation of benzodiazepine (BZD) derivates with different structural characteristics has been studied. Efforts were first focused on the optimization of the analytical conditions, on the effects of buffer concentration, surfactant concentration and buffer pH on the separation. To manipulate selectivity of the separation β-cyclodextrin (CD) was added to the buffer solution, and urea was used to improve the solubility of the CD in water. The CD-modified MEKC separation of the eight BZDs was achieved within 15 minutes using 25 mmol dm −3 natrium tetraborate-50 mmol dm −3 sodium dodecyl sulphate-15 mmol dm −3 β-CD-2 mol dmurea (pH 9.3) as the running buffer. The proposed separation method was evaluated on the basis of precision, linearity and limit of detection.

show abstract

“…Ribavirin (antiviral), Letrozole and Anastrozole (antitumor) are some examples of drugs containing the 1,2,4-triazole moiety [13][14][15][16][17]. 1,3,4-Thiadiazole derivatives are another important class of heterocycles due to their biological activities.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and antimicrobial activity of some new 1,3-thiazoles, 1,3,4-thiadiazoles, 1,2,4-triazoles and 1,3-thiazines incorporating acridine and 1,2,3,4-tetrahydroacridine moieties

Ali

El-Kazak

2010

Eur. J. Chem.

View full text Add to dashboard Cite

Separation of 1,4-benzodiazepines by micellar elektrokinetic capillary chromatography

Cited by 45 publications

References 11 publications

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Separation of 1,4-benzodiazepine Derivates by Micellar Electrokinetic Capillary Chromatography Using Cyclodextrines as Buffer Modifiers

Synthesis and antimicrobial activity of some new 1,3-thiazoles, 1,3,4-thiadiazoles, 1,2,4-triazoles and 1,3-thiazines incorporating acridine and 1,2,3,4-tetrahydroacridine moieties

Contact Info

Product

Resources

About