Separation of 11 protease and reverse transcriptase inhibitors by capillary zone electrophoresis

Abstract

Separation of 11 protease and reverse transcriptase inhibitors by capillary zone electrophoresisCapillary zone electrophoresis is employed for the simultaneous screening of protease and reverse transcriptase inhibitors which are used as antiretroviral therapy drugs against the Human Immunodeficiency Virus. Because of the basic character of the analytes, the electrophoretic system makes use of an acidic buffer electrolyte. In order to establish a strong anodic electroosmotic flow, a cationic surfactant is added to the background electrolyte. This causes the cationic analytes to migrate against the electroosmotic stream (counter-electroosmotic CE). The developed separation system permits a simultaneous separation of protease and reverse transcriptase inhibitors with good selectivity and sensitivity.

Reversed-phase liquid chromatographic method with spectrophotometric detection for the determination of antiretroviral drugs

“…Additionally, MS detection seems to be recommendable for the determination of low concentrations of some PIs as they may present poor absorption in the UV range (e.g., lopinavir) [6,12,[20][21][22]. Apart from HPLC, capillary electrophoresis (CE) has also been used for the determination of drug mixtures in various biological matrices [23][24][25]. It should be remarked that the principal shortcomings of CE methods are the poor detection limits, ranging from 0.2 to 1.0 g mL −1 .…”