Separation of 4-dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine derivatives of carbonyl compounds by reversed-phase capillary electrochromatography

Abstract

4-Dimethylamino-6-(4-methoxy-1-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) is a novel derivatizing reagent specially designed for the determination of carbonyl compounds. In this work, we describe the separation of DMNTH-derivatized carbonyl compounds by reversed-phase capillary electrochromatography (CEC). After systematic investigations of the effects of experimental conditions viz. pH and concentration of buffer, type of stationary phase, injection volume of sample, organic modifier, and temperature, optimal conditions were found. The sample compounds, which were separated with gradient high performance liquid chromatography (HPLC), were separated by CEC under isocratic elution due to the high efficiency. Comparisons of separations by CEC and micellar electrokinetic chromatography (MEKC) were made.

Dendritic Mesoporous Silica Nanospheres: Toward the Ultimate Minimum Particle Size for Ultraefficient Liquid Chromatographic Separation

“…However, to balance the efficiency and the detection sensitivity, the sample plug length is required to be less than 3% of the effective capillary length . Electrokinetic injection is often employed in CEC. − However, electro-injection suffers from injection bias, which is detrimental to the analysis of complex samples containing components with opposite charges. To demonstrate the benefit of good permeability, hydrodynamic injection was used in this study as the CE instrument could provide a pressure of up to 100 psi.…”

Recent progress in adsorbed stationary phases for capillary electrochromatography

“…In HPLC, the peak shape of these compounds can be improved by addition of a competing base such as triethylamine (TEA) to the mobile phase, use of high pH to suppress the protonation of basic compounds, and use of end-capped stationary phases [77]. In CEC, the former two tactics have been employed [78][79][80], but the last one is not applicable due to the EOF requirement. Use of dynamically adsorbed stationary phases of CTAB on bare-silica [35] or SCX packing [36] has proved a useful means to improve the peak shape for basic compounds.…”

The use of hydrazine-based derivatization reagents for improved sensitivity and detection of carbonyl containing compounds using MALDI-MSI

“…Examples are the derivatization of carbonyl containing compounds followed by chromatographic separation and detection by atmospheric pressure chemical ionisation (APCI) mass spectrometry and in water samples [10,11]. The optimised parameters necessary for the separation of DMNTH derivatized carbonyl compounds by reversed phase capillary electrochromatography (CEC) have been described [12].…”