Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography

Schwienheer, C.; Zeiner, Tim; Merz, J.

doi:10.1016/j.jchromb.2015.07.050

Cited by 22 publications

(7 citation statements)

References 29 publications

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“…Moreover, ATPS can also be integrated with other separation techniques such as solvent sublation, which is termed as aqueous two-phase flotation [85, 158]. ATPS has also been successfully integrated with centrifugal partition chromatography (CPC) for the separation of biomolecules [150, 159]. …”

Section: Applications Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

Aqueous two-phase system (ATPS): an overview and advances in its applications

et al. 2016

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Aqueous two-phase system (ATPS) is a liquid-liquid fractionation technique and has gained an interest because of great potential for the extraction, separation, purification and enrichment of proteins, membranes, viruses, enzymes, nucleic acids and other biomolecules both in industry and academia. Although, the partition behavior involved in the method is complex and difficult to predict. Current research shows that it has also been successfully used in the detection of veterinary drug residues in food, separation of precious metals, sewage treatment and a variety of other purposes. The ATPS is able to give high recovery yield and is easily to scale up. It is also very economic and environment friendly method. The aim of this review is to overview the basics of ATPS, optimization and its applications.

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Section: Applications Of Aqueous Two-phase System (Atps)mentioning

confidence: 99%

Aqueous two-phase system (ATPS): an overview and advances in its applications

et al. 2016

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“…It was found that polyoxyethylene n-alkyl ether was an appropriate option for building polymer−salt ATPS because they were composed of two types of diametrically opposed components: polyoxyethylene (hydrophilic) and alkyl (hydrophobic). In our previous papers, 30−37 the polyoxyethylene (10) lauryl ether (POELE10, C 32 H 66 O 11 ) and polyoxyethylene (20) cetyl ether (POELE20, C 56 H 114 O 21 ) were used in the ATPS, and the ATPSs composed of POELE10 and 14 kinds of salt have been given. The phase behaviors of the ATPSs containing POELE20 and six kinds of inorganic salt (KOH, K 2 CO 3 , K 3 PO 4 , Li 2 SO 4 , MgSO 4 , and ZnSO 4 ) have been reported in our articles.…”

Section: Introductionmentioning

confidence: 99%

Measurement and Correlation of Phase Equilibria in Aqueous Two-Phase Systems Containing Polyoxyethylene Cetyl Ether and Three Organic Salts at Different Temperatures

Yang

Sun

et al. 2018

J. Chem. Eng. Data

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The polymer polyoxyethylene(20) cetyl ether (C 56 H 114 O 21 , POELE20) and three salts (K 2 C 2 O 4 , K 2 C 4 H 4 O 6 , and K 3 C 6 H 5 O 7 ) were the phase-forming materials to build the aqueous two-phase system (ATPS) at the temperatures 288.15, 298.15, and 308.15 K. The data of the phase diagram including binodal curve data and tie-line data were determined and fitted by classical equations, and they obtained the satisfactory correlation effect. The phase-forming ability of salt composed of the same cation was investigated by three factors, namely, the valence of the anion, the Gibbs free energy of the anion, and the value of effective excluded volume (EEV). It was found that the phase-forming ability of salt strengthened when the values of said three factors went up. The results of the analysis on the data from the present work and that in our previous paper showed that the order of the phase-forming ability of salts was KOH

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“…Cerrena unicolor (Wang et al, 2017), Lentinula edodes (Nagai et al, 2003), Pycnoporus sanguineus (Ramírez-Cavazos et al, 2014), Trametes orientalis (Zheng et al, 2017), Trichoderma harzianum (El Monssef et al, 2016;Bagewadi et al, 2017), Pleurotus ostreatus (Othman et al, 2014;El-Batal et al, 2015), Pleurotus sajor-caju (Fokina et al, 2016), Pleurotus pulmonarius (Marques de Souza and Peralta, 2003;Behrens et al, 2017), Pleurotus sapidus (Schwienheer et al, 2015), Pleurotus eryngii (Loi et al, 2018), Pleurotus ferulae (Ding et al, 2014;Wang et al, 2015), and Pleurotus djamor (Salmones and Mata 2015) have been purified, characterized and studied in detail by many authors. However, laccase produced by P. tuber-regium has never been studied in detail, even though a previous study demonstrated the versatility of this fungus (Isikhuemhen and Nerud, 1999).…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of laccase from Pleurotus tuber-regium and its application in dye decolourization

Bamigboye¹,

Oloke²,

Olaogun³

et al. 2019

bta

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Pleurotus tuber-regium is a unique sclerotium-forming white rot fungus. It is edible and has medicinal value and a high potentials of application in various industries. However, to date, there is no information on the production of laccase by this fungus, the purification and determination of decolourization potential of laccase have also not been reported. In this study, purification of laccase from P. tuber-regium is demonstrated for the first time. Laccase was purified from the submerged culture of P. tuber-regium using ammonium sulfate precipitation, ion-exchange chromatography and gel filtration chromatography. The molecular weight of laccase was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified laccase was characterized to determine its optimum pH and temperature, substrate specificity, Michaelis-Menten constant, and inhibitors. The decolourization potential of the crude laccase was evaluated using Congo red, trypan blue, textile dyes and textile effluent. The purified laccase had a molecular weight of 52 kDa, optimum pH and temperature were 4 and 60EC, respectively. The best substrate for laccase was found to be 2,2N-azino-bis (3-ethylbenzothiazoline-6-sulfonate), with a Michaelis-Menten constant (K m ) of 7.82 μM. Laccase activity was mildly inhibited by ethylenediamine tetraacetic acid (EDTA) and L-cysteine, but strongly inhibited by sodium azide. The activity of the purified laccase was enhanced by more than two fold by Na + , Ca 2+ and Ba 2+ , and slightly enhanced by Hg 2+ and Mn 2+ . The crude laccase was able to efficiently decolourize trypan blue and Congo red dyes and red and blue textile dyes. Notably, on solid agar, trypan blue was completely degraded by intracellular laccase.

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Separation of active laccases from Pleurotus sapidus culture supernatant using aqueous two-phase systems in centrifugal partition chromatography

Cited by 22 publications

References 29 publications

Aqueous two-phase system (ATPS): an overview and advances in its applications

Aqueous two-phase system (ATPS): an overview and advances in its applications

Measurement and Correlation of Phase Equilibria in Aqueous Two-Phase Systems Containing Polyoxyethylene Cetyl Ether and Three Organic Salts at Different Temperatures

Purification and characterization of laccase from Pleurotus tuber-regium and its application in dye decolourization

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