Separation of Amino Acids Based on Thin-Layer Chromatography by a Novel Quinazoline Based Anti-Microbial Agent

Abstract

A newly designed quinazoline based compound, 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline (PDBIQ) has shown the ability for the easy detection of nineteen amino acids on thin-layer chromatography plates as a spray reagent. This new reagent enabled to produce various distinguishable colors with amino acids with different R<sub>F</sub> values. The detection limits and the binding ability of PDBIQ with amino acids have been calculated. PDBIQ is also able to detect aminoacids from hydrolised seed protein. The title compound also exhibited profound inhibitory action against some gm (+ve) and gm (-ve) bacterial organisms. This paper deals with synthesis, spectroscopic application and biological evaluation of the organic moity

Effect of plant growth promoting Bacillus spp. on nutritional properties of Amaranthus hypochondriacus grains

“…Presence of all three test amino-acids (lysine, methionine, and tryptophan) in Amaranthus hypochondriacus grains obtained by different bacilli treatments was evidenced by TLC. Similar study was carried out by Sen et al (2012) who reported the presence of different amino-acids in the seeds of Amanita excelsa . Inoculation of PGPRs has been reported very effective to enhance the amino-acid contents of various crops such as maize ( Hamdia et al, 2004 ).…”

Antibacterial Activity of The Fungal Metabolite Trichoderma longibrachiatum against Multidrug-Resistant Klebsiella pneumoniae and Methicillin-Resistant Staphylococcus aureus

“…Spot identification using the value of R f (Retention Factor) and spraying with staining reagents. After the elution process, TLC was visualized by UV light (365 nm), 2% vanillin-H 2 SO 4 , 0.25% ninhydrin in acetone, and 1% ferric (III) chloride in methanol (Harborne, 1984;Sen et al, 2012;Trianto et al, 2019). Furthermore, the TLC plate was heated at 110 o C for 2-3 minutes.…”

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“…Spot identification using the value of R f (Retention Factor) and spraying with staining reagents. After the elution process, TLC was visualized by UV light (365 nm), 2% vanillin-H 2 SO 4 , 0.25% ninhydrin in acetone, and 1% ferric (III) chloride in methanol (Harborne, 1984;Sen et al, 2012;Trianto et al, 2019). Furthermore, the TLC plate was heated at 110 o C for 2-3 minutes.…”