Separation of astaxanthin from cells of Phaffia rhodozyma using colloidal gas aphrons in a flotation column

Abstract

The aim of this study is to investigate the separation of astaxanthin from the cells of Phaffia rhodozyma using colloidal gas aphrons (CGA), which are surfactant stabilized microbubbles, in a flotation column. It was reported in previous studies that optimum recoveries are achieved at conditions that favor electrostatic interactions. Therefore, in this study, CGA generated from the cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB) were applied to suspensions of cells pretreated with NaOH. The different operation modes (batch or continuous) and the effect of volumetric ratio of CGA to feed, initial concentration of feed, operating height, and flow rate of CGA on the separation of astaxanthin were investigated. The volumetric ratio was found to have a significant effect on the separation of astaxanthin for both batch and continuous experiments. Additionally, the effect of homogenization of the cells on the purity of the recovered fractions was investigated, showing that the homogenization resulted in increased purity. Moreover, different concentrations of surfactant were used for the generation of CGA for the recovery of astaxanthin on batch mode; it was found that recoveries up to 98% could be achieved using CGA generated from a CTAB solution 0.8 mM, which is below the CTAB critical micellar concentration (CMC). These results offer important information for the scale-up of the separation of astaxanthin from the cells of P. rhodozyma using CGA.

Surfactant TWEEN20 provides stabilisation effect on anthocyanins extracted from red grape pomace

“…A surfactant-based separation technique, colloidal gas aphrons (CGA) has been previously studied in our group to recover various valuable bioactive compounds from 4 different feedstock such as astaxanthin (Dermiki, Bourquin, & Jauregi, 2010;Dermiki, Gordon, & Jauregi, 2009), proteins (Fuda & Jauregi, 2006;Fuda, Bhatia, Pyle, & Jauregi, 2005) and polyphenols (MohdMaidin, Michael, Oruna-Concha, & Jauregi, 2017;Spigno, Dermiki, Pastori, Casanova, & Jauregi, 2010;Spigno, Amendola, Dahmoune, & Jauregi, 2015). The type of surfactant (i.e cationic, anionic and non-ionic) determines the outer charge of the CGA, where molecules with the opposite charge will attract to the CGA resulting in their effective separation into the CGA phase.…”

Exploring different strategies of separation of antioxidant compounds from winery by-products via surfactant-assisted processes for process intensification and integration

“…CGAs obtained from solutions of Tween20 surfactant at 1 mM, 10 mM, and 20 mM (200 mL), were characterized for stability in terms of gas hold up () and half-life (t 1/2 ) in the generation vessel (Jauregi and Dermiki, 2010). Higher surfactant concentrations were not tested since, as similarly reported by Dermiki et al (2010), no additional effects on CGAs structure were observed. For the experiments, freshly produced CGAs were transferred into a 500 mL volumetric cylinder and the volume of drained liquid was registered at regular time intervals within the range 0-25 min, until complete foam collapse.…”

Astaxanthin Production by Newly Isolated Rhodosporidium toruloides: Optimization of Medium Compositions by Response Surface Methodology

“…Yeast has been currently suggested as an alternative for astaxanthin production because of its rapid growth rate, high biomass productivity, and health benefits (Ukibe et al, 2009;Freitas et al, 2014 b;Lin et al, 2017 b). Phaffia rhodozyma (Xanthophyllomyces dendrorhous) is a red-pigmented heterobasidiomycetous yeast, which has been extensively studied for astaxanthin production (Domínguez-Bocanegra et al, 2007;Dermiki et al, 2010;Stoklosa et al, 2018). However, low astaxanthin productivity in wild strain P. rhodozyma limits its industrial application (Fang and Cheng, 1993;Domínguez-Bocanegra et al, 2007;Liu et al, 2008;Montanti et al, 2011).…”