Separation of B‐ and T‐lymphocytes by cellular adsorption chromatography using poly(2‐hydroxyethyl methacrylate)/polyamine graft copolymer as column adsorbent

Maruyama, Atsushi; Tsuruta, Teiji; Kataoka, Kazunori; Sakurai, Yasuhisa

doi:10.1002/jbm.820220610

Cited by 24 publications

(4 citation statements)

References 17 publications

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“…4 There is also a large demand for T and B cell separation in the therapy and diagnosis of autoimmune disease and cancer. [5][6][7][8] Specific T cell selection (separation), which has ability of GVL (graft-versus-leukemia effect) but does not have ability of GVHD will enable the clinical application of lymphocyte therapy, because the T cells responsible to GVL and GVHD may be different. 9 Blood cell separation is typically performed by centrifugation, fluorescence activated cell sorting (FACS), 9 magnetic cell selection, 3,10 -12 or membrane filtration.…”

Section: Introductionmentioning

confidence: 99%

Peripheral blood cell separation through surface‐modified polyurethane membranes

Higuchi

Yamamiya

Yoon

et al. 2003

J Biomedical Materials Res

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Cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with different functional groups. Both red blood cells and platelets could pass through unmodified PU and PU-SO 3 H membranes, whereas the red blood cells preferentially passed through PU-N(C 2 H 5 ) 2 and PU-NHC 2 H 4 OH membranes. The permeation ratio of T and B cells was Ͻ25% for the surface-modified and unmodified PU membranes. CD34 ϩ cells have been recognized as various kinds of stem cells including hematopoietic and mesenchymal stem cells. The adhesiveness of CD34 ϩ cells on the PU membranes was found to be higher than that of red blood cells, platelets, T cells, or B cells. Overall, the adhesiveness of blood cells on the PU membranes increased in the following order: red blood cells Յ platelets Ͻ T cells Յ B cells Ͻ CD34 ϩ cells. Treatment of PU-COOH membranes with a human albumin solution to detach adhered blood cells, allowed recovery of mainly CD34 ϩ cells in the permeate, whereas both red blood cells and platelets could be isolated in the permeate using unmodified PU membranes. The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes.

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Section: Introductionmentioning

confidence: 99%

Peripheral blood cell separation through surface‐modified polyurethane membranes

Higuchi

Yamamiya

Yoon

et al. 2003

J Biomedical Materials Res

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“…Amino group (NH 2 ) was introduced from opening reaction of epoxy group on the PU‐epoxy membranes, which were following by the reaction between epoxy group and NH 3 reported in the literature 23, 24. PU‐epoxy membranes were immersed in 0.5 mol/L of aqueous NH 3 solution containing 0.1 mol/L of NaOH at 303 K for 6 h. The anticipated product by the ring‐opening reaction of epoxy group is shown in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes

Higuchi¹,

Sekiya²,

Gomei³

et al. 2007

J Biomedical Materials Res

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Cell separation from peripheral blood was investigated using polyurethane (PU) foam membranes having 5.2 mum pore size and coated with Pluronic F127 or hyaluronic acid. The permeation ratio of hematopoietic stem cells (CD34(+) cells) and lymphocytes through the membranes was lower than for red blood cells and platelets. Adhered cells were detached from membrane surfaces using human serum albumin (HSA) solution after permeation of blood through the membranes, allowing isolation of CD34(+) cells in the permeate (recovery) solution. High-yield isolation of CD34(+) cells was achieved using Pluronic-coated membranes. This was because the Pluronic coating dissolved into the recovery solution at 4 degrees C, releasing adhered cells from the surfaces of the membranes during permeation of HSA solution through these membranes. Dextran and/or bovine serum albumin solutions were also evaluated for use as recovery solutions after blood permeation. A high recovery ratio of CD34(+) cells was achieved at 4 degrees C in a process using 20% dextran solution through PU membranes having carboxylic acid groups.

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“…Poly-L-lysine is widely used as a culture substrate to enhance cell adhesion, because electrostatic attractions between negatively charged cell surfaces and positively charged amino groups mediate interactions. The separation of B-cells from lymphocyte subpopulations was successfully achieved using polyamine-graft-poly(2-hydroxyethyl methacrylate) copolymer surface [1]. Additionally, microheterogeneous structures on material surfaces have been shown to inhibit blood platelet adhesion and aggregation in thrombosis.…”

Section: Introductionmentioning

confidence: 97%

Endothelial cell differentiation into capillary structures by copolymer surfaces with phenylboronic acid groups

Aoki

Nagao

Terada

et al. 1996

Journal of Biomaterials Science, Polymer Edition

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A ternary copolymer composed of m-acrylamidophenylboronic acid, N,N-dimethylaminopropylmethacrylamide and N-isopropylacrylamide was synthesized. Long-term culture of bovine aortic endothelial cells on this copolymer substrate demonstrated adhesion and proliferation of the cells. After 26 days in culture, endothelial cells spontaneously developed into capillary networks. The interactions between phenylboronic acid groups in copolymer and glycoconjugates on endothelial cell plasma membranes are proposed to regulate the induction of tissue formation, since phenylboronic acid groups are known to specifically form reversible complexes with cis-diol compounds such as glucose. This copolymer is a novel material capable of mediating specific signals analogous to extracellular matrix to promote proliferation of endothelial cells, inducing capillary structures and prompt angiogenesis.

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Separation of B‐ and T‐lymphocytes by cellular adsorption chromatography using poly(2‐hydroxyethyl methacrylate)/polyamine graft copolymer as column adsorbent

Cited by 24 publications

References 17 publications

Peripheral blood cell separation through surface‐modified polyurethane membranes

Peripheral blood cell separation through surface‐modified polyurethane membranes

Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes

Endothelial cell differentiation into capillary structures by copolymer surfaces with phenylboronic acid groups

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