Separation of bacteria with imprinted polymeric films

Schirhagl, Romana; Hall, Eric W.; Fuereder, Ingo; Zare, Richard N.

doi:10.1039/c2an15927a

Cited by 62 publications

(63 citation statements)

References 31 publications

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“…To investigate the surface property of Δ lprG and determine the role of LprG in expression of LAM on the surface [10], we employed cell-imprinting technology [25], [26]. As illustrated in Figure 2A, cell-imprinting cures PDMS polymer solution around bacteria of interest.…”

Section: Resultsmentioning

confidence: 99%

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LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis

et al. 2014

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Mycobacterium tuberculosis employs various virulence strategies to subvert host immune responses in order to persist and cause disease. Interaction of M. tuberculosis with mannose receptor on macrophages via surface-exposed lipoarabinomannan (LAM) is believed to be critical for cell entry, inhibition of phagosome-lysosome fusion, and intracellular survival, but in vivo evidence is lacking. LprG, a cell envelope lipoprotein that is essential for virulence of M. tuberculosis, has been shown to bind to the acyl groups of lipoglycans but the role of LprG in LAM biosynthesis and localization remains unknown. Using an M. tuberculosis lprG mutant, we show that LprG is essential for normal surface expression of LAM and virulence of M. tuberculosis attributed to LAM. The lprG mutant had a normal quantity of LAM in the cell envelope, but its surface was altered and showed reduced expression of surface-exposed LAM. Functionally, the lprG mutant was defective for macrophage entry and inhibition of phagosome-lysosome fusion, was attenuated in macrophages, and was killed in the mouse lung with the onset of adaptive immunity. This study identifies the role of LprG in surface-exposed LAM expression and provides in vivo evidence for the essential role surface LAM plays in M. tuberculosis virulence. Findings have translational implications for therapy and vaccine development.

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Section: Resultsmentioning

confidence: 99%

“…Cell-imprinting was performed as previously described [25], [26]. Briefly, one drop of bacteria in PBS at an OD 580 of 2 was placed on a polystyrene slide (Evergreen) and incubated overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis

et al. 2014

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“…In the presented case, we believe that the main binding mechanism is governed by the formation of hydrogen bonds and by hydrophobic interactions. This is in agreement with what has been reported for different biosamples [37][38][39]. At the same time the polymer is crosslinked which guarantees that the polymeric cavity preserves the shape of the antibody.…”

Section: Double Imprintingsupporting

confidence: 81%

Immunosensing with artificial antibodies in organic solvents or complex matrices

Schirhagl

Qian

Dickert

2012

Sensors and Actuators B: Chemical

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a b s t r a c tThe detection of analytes in complex matrices without labour intensive sample preparation is an important goal in analytical chemistry. In this article we would like to address this issue by transferring the selectivity of natural antibody in a cheap, robust and reusable polymer, employing a double imprinting protocol. Antibodies with the desired selectivity were used as template to generate imprinted polymer particles. These antibodies were added to a prepolymer and particles were precipitated. After the antibodies were removed from the particles, cavities remained which reproduce size, shape and surface chemistry of the antibodies. In a second imprinting step the particles with cavities were pressed into a second polymer. After the second polymer has cured the particles can be removed leaving positive structures behind that react with the desired antigen. Such a sensitive coating was applied to the surface of a quartz crystal microbalance and incorporated into a microfluidic chip. An immunosensor for estradiol was fabricated having six times higher affinity to its antigen than to structurally related molecules. The measurements can also be performed after an extraction into an organic solvent which improves the detection limit greatly and would not be possible for natural antibodies. The feasibility of the method for complex matrices was shown by detecting viruses in plasma or allergenic protein in bread extract.

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“…The literature reports the development of MI membranes able to recognize uric acid [93], low density lipoproteins and cholesterol [94,95] as proof of concept of the final application. Cell-imprinted membranes were also reported for the retention of bacteria [96,97].…”

Section: Membranes: Smart Separation Of Single Molecules and Tissue Engmentioning

confidence: 97%

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

Gagliardi

Mazzolai

2015

Future Med. Chem.

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Molecular imprinting (MI) represents a strategy to introduce a 'molecular memory' in a polymeric system obtaining materials with specific recognition properties. MI particles can be used as drug delivery systems providing a targeted release and thus reducing the side effects. The introduction of molecular recognition properties on a polymeric drug carrier represents a challenge in the development of targeted delivery systems to increase their efficiency. This review will summarize the limited number of drug delivery MI particles described in the literature along with an overview of potential solutions for a larger exploitation of MI particles as targeted drug delivery carriers. Molecularly imprinted drug carriers can be considered interesting candidates to significantly improve the efficiency of a controlled drug treatment.

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Separation of bacteria with imprinted polymeric films

Cited by 62 publications

References 31 publications

LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis

LprG-Mediated Surface Expression of Lipoarabinomannan Is Essential for Virulence of Mycobacterium tuberculosis

Immunosensing with artificial antibodies in organic solvents or complex matrices

Molecularly Imprinted Polymeric Micro- and Nano-Particles for the Targeted Delivery of Active Molecules

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