Separation of barbiturates with micellar liquid chromatography and optimization by a second order mathematical design

Rukhadze, M. D.; Bezarashvili, George; Sebiskveradze, Maya V.; Meyer, Veronika R.

doi:10.1016/s0021-9673(97)01301-0

Cited by 20 publications

(4 citation statements)

References 12 publications

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“…For better understanding of the typical factors which govern the retention, a complementary set of experiments as fold-over design was performed (Table II, experiments [13][14][15][16][17][18][19][20]. This represents a fold-over of the previous design and is obtained by changing the sign of the three last columns of the fractional design.…”

Section: Fold-over Fractional Factorial Designmentioning

confidence: 99%

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Chemometrically optimized micellar liquid chromatographic method for the simultaneous determination of cetirizine dihydrochloride in its combined dosage forms. Application to biological fluids and pharmacokinetic studies

El-Din¹,

Ibrahim²,

Eid³

et al. 2013

Acta Chromatographica

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Abstract. An accurate, simple, sensitive, and selective micellar liquid chromatographic method has been developed for the simultaneous determination of cetirizine dihydrochloride in two of its combined pharmaceutical preparations with psudoephedrine hydrochloride and/or paracetamol. The chemometrics approach was applied for the optimization of separation of the studied drugs to optimize their separation; the effect of six experimental parameters on retention was investigated by means of multivariate analysis. Separation was conducted using an ODS C18 (150 × 4.6 mm id) stainless steel column at ambient temperature with UV-detection at 250 nm. A mobile phase composed of 0.135 M sodium dodecyl sulphate, 11% 1-propanol, 0.3% tri-ethylamine in 0.02 M phosphoric acid, and adjusted to pH 3.3 has been used at a flow rate of 1 mL/min. Regression models were characterized by both descriptive and predictive ability (R 2 ≥ 98.8% and R 2 cv ≥ 94.5%) and allowed the chromatographic separation of the drugs with a good resolution and a total analysis time within 6 min.The calibration curves were rectilinear over the concentration ranges of 0.05-1.0, 0.07-4.0, and 1.0-10.0 μg/mL for cetirizine dihydrochloride, psudoephedrine hydrochloride, and paracetamol respectively; with detection limits of 0.01, 0.05, and 0.08 μg/mL, and quantification limits of 0.04, 0.09, and 1.02 μg/mL, respectively. The results obtained were in good agreement with those obtained by the comparison method. Furthermore, the method was applied for the determination of the drugs in spiked human plasma and used to reveal the pharmacokinetic characters in a healthy volunteer treated with oral administration of the studied two combined dosage forms of cetirizine.

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Section: Fold-over Fractional Factorial Designmentioning

confidence: 99%

“…Variations of the experimental conditions affect both the retention of the analytes and the extent of the surfactant monomers adsorbed onto the stationary phase. As a result, the effect of these factors on retention can be interdependent and nonlinear [14][15][16]. Therefore, methods of multivariate analysis are usually used.…”

Section: Introductionmentioning

confidence: 99%

Chemometrically optimized micellar liquid chromatographic method for the simultaneous determination of cetirizine dihydrochloride in its combined dosage forms. Application to biological fluids and pharmacokinetic studies

El-Din¹,

Ibrahim²,

Eid³

et al. 2013

Acta Chromatographica

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show abstract

“…Furthermore, due to the dependence on a large number of factors including the mobile phase composition, percentage of organic modifier, flow rate and temperature, optimization of the chromatographic conditions is a complicated process in RP-HPLC. On the other hand, since the effect of these factors on retention can be interdependent and nonlinear the systematic approach for optimization of chromatographic separations is more expedient for such complicated method [17,18].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous optimization of the resolution and analysis time of flavonoids in reverse phase liquid chromatography using Derringer's desirability function

Hadjmohammadi¹,

Sharifi²

2012

Journal of Chromatography B

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“…As a result, the effect of these factors on retention can be interdependent and nonlinear. Therefore, prediction of chromatographic behavior, which should lead to an improved separation, is a complicated process in MLC [10][11][12][13][14][15][16][17]. For this purpose, methods of multivariate analysis are usually used.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the separation of coumarins in mixed micellar liquid chromatography using Derringer's desirability function

Ebrahimi

Hadjmohammadi

2007

Journal of Chemometrics

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The multi-criteria decision making (MCDM) method was applied for the optimization of the separation of eight coumarin compounds in mixed micellar liquid chromatography (MLC). In order to achieve this goal the usefulness of Derringer's desirability function for the evaluation of the two different chromatographic performance goals (resolution and analysis time) was tested. The effect of six experimental parameters on a chromatographic response function (CRF) expressed as a product of two sigmoidal desirability functions was investigated. The sigmoidal functions were used to transform the optimization criteria, resolution and analysis time, into the desirability values. The factors studied were the concentration of sodium dodecyl sulfate (SDS) and polyxyethylene (23) dodecanol (Brij-35), alkyl chain length of the alcohol used as the organic modifier, organic modifier concentration, mobile phase pH and temperature. For the development of the method and the optimization of the chromatographic conditions, the experiments were performed according to a face-centered cube response surface experimental design. The qualities of the obtained chromatograms were calculated using CRF and model was built by the use of MLR to correlate the obtained CRFs to the variables and their interactions. The MLR model developed showed good descriptive and predictive ability (R 2 ¼ 0.998, F ¼ 626.056, SE ¼ 0.009, R 2 CV ¼ 0.985) and was used, by a grid search algorithm, to optimize the chromatographic conditions for the separation of the mixture. The efficiency of prediction of polynomial model was confirmed by performing the experiment under the optimal conditions. The results of the study indicated that a CRF composed of sigmoid transformations can also be successfully used for the evaluation of the chromatograms.

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Separation of barbiturates with micellar liquid chromatography and optimization by a second order mathematical design

Cited by 20 publications

References 12 publications

Chemometrically optimized micellar liquid chromatographic method for the simultaneous determination of cetirizine dihydrochloride in its combined dosage forms. Application to biological fluids and pharmacokinetic studies

Chemometrically optimized micellar liquid chromatographic method for the simultaneous determination of cetirizine dihydrochloride in its combined dosage forms. Application to biological fluids and pharmacokinetic studies

Simultaneous optimization of the resolution and analysis time of flavonoids in reverse phase liquid chromatography using Derringer's desirability function

Optimization of the separation of coumarins in mixed micellar liquid chromatography using Derringer's desirability function

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