Separation of basic central nervous system drugs by capillary electrochromatography

Abstract

Separation of basic central nervous system drugs by capillary electrochromatographyThis article describes the sample preparation and separation of three strongly basic central nervous system (CNS) drugs by capillary electrochromatography. The separation was developed on Hypersil C8 MOS and Hypersil Phenyl stationary phases using acetonitrile (ACN) as organic modifier together with mobile phase additives, ammonia, ethylenediamine, or 1,3-diaminopropane. A successful, fast separation after a simple derivatization procedure of the compound was achieved on the Hypersil Phenyl stationary phase using ethylenediamine as mobile phase additive. The general approach to method development for complex mixtures is discussed.

Investigation of a novel mixed‐mode stationary phase for capillary electrochromatography. Part III: Separation of nucleosides and nucleic acid bases on sulfonated naphthalimido‐modified silyl silica gel

“…The influence of the buffer concentration on the separation was investigated at three phosphate buffer concentrations (10,20, and 30 mM) in the mobile phase containing 40% (v/v) methanol while keeping pH at 3.8. The EOF mobility was not affected by the change of buffer concentration.…”

Recent applications in capillary electrochromatography

“…Jiskra et al [96] described a method for the separation of central nervous system drugs after derivatization to transform a zwitterionic drug into a basic form, using Hypersil MOS C 8 and Hypersil phenyl stationary phases. Basic additives were added to the mobile phase for shielding.…”

Separation of basic compounds by capillary electrochromatography on an X-Terra RP18® stationary phase

“…The main problem encountered with the analysis of basic compounds is due to the interaction between their basic nitrogen and the residual silanol groups of the packing materials. To date, the methodology usually used to analyse basic compounds by electrochromatography relies on the addition of small competiting bases such as triethylamine [5], triethanolamine [5], hexylamine [6], ethylenediamine [7,8] in the mobile phases although this addition slows down the EOF. An other approach to analyse basic compounds has also been described by several authors using unbonded silica in CEC with simple aqueous-organic mobile phase with or without the need for competiting bases in the mobile phase, with good efficiencies and separation performance [9][10][11][12][13][14].…”