2019
DOI: 10.1101/2019.12.23.887711
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Separation of Bioactive Small Molecules, Peptide from Natural Products and Proteins from Pathogenic Microbe by Rotofor-based Preparative Isoelectric Focusing (IEF) Method

Abstract: The primary purpose of purifying the bioactive small molecules is obtaining enough amount of specific molecules for their downstream applications. There are various methods available for serving this purpose. However, not all can afford for cost-effective purification methods, especially for complex biological samples. Here we demonstrate, the Rotofor based preparative IEF separation which shows the high possibility of separating and enriching desired molecules, including small molecules and peptides from the … Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2021
2021
2021
2021

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 16 publications
0
1
0
Order By: Relevance
“…The peptide fraction with the highest antimicrobial and antioxidant activity was selected and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis was performed on this fraction as described by Veerapandian et al (2020). Fifteen percent polyacrylamide gel was used in the Mini-Protean III system.…”
Section: Isolation and Purification Of Bioactive Peptidesmentioning
confidence: 99%
“…The peptide fraction with the highest antimicrobial and antioxidant activity was selected and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis was performed on this fraction as described by Veerapandian et al (2020). Fifteen percent polyacrylamide gel was used in the Mini-Protean III system.…”
Section: Isolation and Purification Of Bioactive Peptidesmentioning
confidence: 99%