Separation of CD34<sup>+</sup> cells from human peripheral blood through polyurethane foaming membranes

Higuchi, Akon; Iizuka, Ayumi; Gomei, Yumiko; Miyazaki, Toshihisa; Sakurai, Masaru; Matsuoka, Yuki; Natori, Shizue

doi:10.1002/jbm.a.30729

Cited by 16 publications

(32 citation statements)

References 27 publications

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“…The blood (feed solution) was subsequently injected into the Syringe 2 described in a previous study. [7][8][9] Two syringes (Syringe 1 and Syringe 2) were attached by head to head. Water was injected into Syringe 1 using perister pump (MP-3, Tokyo Rikakiki Co.) at the speed of 0.3 mL/ min, and therefore, head of Syringe 1 pushed back Syringe 2 containing blood subsequently.…”

Section: Blood Cell Permeationmentioning

confidence: 99%

“…In this study, we prepared not only the PUACOOH membranes used in our previous studies, [7][8][9] but also different types of PUACOOH membranes modified with several amino acids, and conducted further experiments on the separation of CD34 þ cells using these modified membranes. Furthermore, we used a variety of experimental conditions in the permeation and recovery processes, and investigated the effect of permeation volume, type, and concentration of recovery solution on the detachment of CD34 þ cells (i.e., recovery ratio of CD34 þ cells) during the recovery process at 25 C.…”

Section: Introductionmentioning

confidence: 99%

“…23 Therefore, the transplantation of CD34 þ cells is required in the therapy of patients with acute myeloid leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and systemic mastocytosis. 24 In previous investigations, [7][8][9] cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with a pore size of 5 lm, carrying different functional groups. CD34 þ cells adhered more strongly to the membranes than red blood cells, platelets, T cells, or B cells.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9] Efficient cell separation is an important issue for the successful isolation and purification of blood cells, stem cells, and specific tissue cells. Centrifugation, 10,11 affinity column chromatography, 12,13 fluorescence-activated cell sorting (FACS), 14,15 magnetic cell selection (MACS), 16,17 and membrane filtration [7][8][9][18][19][20] are techniques typically employed for cell separation. Highly purified cellular preparations are obtained using FACS or MACS in conjunction with a fluorescently labeled antibody as the cell-surface marker, whereas the centrifugal separation of cells is commonly used to isolate platelets, leukocytes, mononuclear cells, red blood cells, and non-blood cells.…”

Section: Introductionmentioning

confidence: 99%

“…We also investigated blood cell permeation through unmodified PU and PU membranes carrying carboxylic acid groups (PUACOOH) with pore sizes of either 5.2 or 12 lm at different blood permeation speeds. 8 The most highly purified CD34 þ cells (highest recovery ratio of CD34 þ cells to red blood cells) were obtained in a recovery process using PUACOOH membranes with pore size r ¼ 5.2 lm at blood permeation speeds of 0.3 mL/min to 1 mL/min. 8 We also developed different kinds of filtration membranes such as Pluronic-coated and Pluronic-immobilized membranes.…”

Section: Introductionmentioning

confidence: 99%

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Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Higuchi

Yang

et al. 2009

J of Applied Polymer Sci

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The separation of hematopoietic stem and progenitor cells (CD34 þ cells) from peripheral blood was investigated using foamed polyurethane (PU) membranes modified with several amino acids. CD34 þ cells were collected by first allowing the blood to permeate through the membranes, and then passing the recovered solution through the membranes. Optimal conditions for the separation of CD34 þ cells were investigated. The highest recovery ratio of CD34 þ cells was obtained using three sheets of PU membranes having carboxylic acid groups (PUACOOH) modified with glycine, the membranes having been pretreated by immersion in phosphate buffer solution prior to permeation of blood. A high recovery ratio of CD34 þ cells was achieved in a recovery process using 0.5 wt % human serum albumin (HSA) or 20% dextran solution passed through PUACOOH membranes. The recovery ratios of CD34 þ cells using platelet-poor plasma and platelet-rich plasma were approximately 20% and 30%, respectively, significantly less than the ratio found using 0.5 wt % HSA solution. Surface-modified membranes having carboxylic acid groups showed a higher recovery ratio of CD34 þ cells than membranes having zwitterionic groups. The effect of carboxylic acid groups on the surface-modified PU membranes was to generate weak interactions by electrostatic repulsion between CD34 þ cells and the membranes because of the negatively charged surfaces of the cells, allowing them to be detached from the membranes and collected in the recovered solution.

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Section: Blood Cell Permeationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Higuchi

Yang

et al. 2009

J of Applied Polymer Sci

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Separation technologies for stem cell bioprocessing

Diogo

Silva

Cabral

2012

Biotech & Bioengineering

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Stem cells have been the focus of an intense research due to their potential in Regenerative Medicine, drug discovery, toxicology studies, as well as for fundamental studies on developmental biology and human disease mechanisms. To fully accomplish this potential, the successful application of separation processes for the isolation and purification of stem cells and stem cell-derived cells is a crucial issue. Although separation methods have been used over the past decades for the isolation and enrichment of hematopoietic stem/progenitor cells for transplantation in hemato-oncological settings, recent achievements in the stem cell field have created new challenges including the need for novel scalable separation processes with a higher resolution and more cost-effective. Important examples are the need for high-resolution methods for the separation of heterogeneous populations of multipotent adult stem cells to study their differential biological features and clinical utility, as well as for the depletion of tumorigenic cells after pluripotent stem cell differentiation. Focusing on these challenges, this review presents a critical assessment of separation processes that have been used in the stem cell field, as well as their current and potential applications. The techniques are grouped according to the fundamental principles that govern cell separation, which are defined by the main physical, biophysical, and affinity properties of cells. A special emphasis is given to novel and promising approaches such as affinity-based methods that take advantage of the use of new ligands (e.g., aptamers, lectins), as well as to novel biophysical-based methods requiring no cell labeling and integrated with microscale technologies.

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Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes

Higuchi¹,

Sekiya²,

Gomei³

et al. 2007

J Biomedical Materials Res

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Cell separation from peripheral blood was investigated using polyurethane (PU) foam membranes having 5.2 mum pore size and coated with Pluronic F127 or hyaluronic acid. The permeation ratio of hematopoietic stem cells (CD34(+) cells) and lymphocytes through the membranes was lower than for red blood cells and platelets. Adhered cells were detached from membrane surfaces using human serum albumin (HSA) solution after permeation of blood through the membranes, allowing isolation of CD34(+) cells in the permeate (recovery) solution. High-yield isolation of CD34(+) cells was achieved using Pluronic-coated membranes. This was because the Pluronic coating dissolved into the recovery solution at 4 degrees C, releasing adhered cells from the surfaces of the membranes during permeation of HSA solution through these membranes. Dextran and/or bovine serum albumin solutions were also evaluated for use as recovery solutions after blood permeation. A high recovery ratio of CD34(+) cells was achieved at 4 degrees C in a process using 20% dextran solution through PU membranes having carboxylic acid groups.

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Separation of CD34⁺ cells from human peripheral blood through polyurethane foaming membranes

Cited by 16 publications

References 27 publications

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Separation technologies for stem cell bioprocessing

Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes

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Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes

Cited by 16 publications

References 27 publications

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Separation of hematopoietic stem and progenitor cells from human peripheral blood through polyurethane foaming membranes modified with several amino acids

Separation technologies for stem cell bioprocessing

Separation of hematopoietic stem cells from human peripheral blood through modified polyurethane foaming membranes

Contact Info

Product

Resources

About

Separation of CD34⁺ cells from human peripheral blood through polyurethane foaming membranes