Separation of cells by affinity chromatography on SpA-sepharose 6MB

Gheţie, Victor; Moţa, Gabriela; Sjöquist, John

doi:10.1016/0022-1759(78)90230-2

Cited by 55 publications

(10 citation statements)

References 7 publications

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“…Although affinity chromatography of cells is a rather old method, it has never been widely accepted due to the complexity of the process and the low capacity of conventional packed beds [72]. It appears now that superporous monolithic cryogels may give a boost to this method.…”

Section: Cryogel As Adsorbents For Bioseparation and Bioconversionmentioning

confidence: 99%

Monoliths for fast bioseparation and bioconversion and their applications in biotechnology

Jungbauer¹,

Hahn

2004

J of Separation Science

161

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Monoliths have consolidated their position in bioseparation. More than 200 different applications have been reported in the past two decades and their advantages compared to conventional chromatography demonstrated. These include the high mass transfer efficiency due to the convective flow enabled by the macroporous character of the matrix. Recently plasmid DNA and viruses were separated with high efficiency and cryogels and monolithic superporous agarose were developed for capture of proteins from crude homogenates and separation of microorganisms or lymphocytes. Currently four companies manufacture monoliths mainly for analytical applications although monoliths with a volume of 0.8 liter are commercially available and 8 L are available as prototypes. A book entitled "Monolithic materials: preparation, properties and applications" was published in 2003 and became standard reference of the status of this area. This review focuses on the progress in monoliths that goes beyond the scope of this reference book. Less progress has been made in the field of bioconversions in spite of the fact that monolithic supports exhibit better performance than beads in enzymatic processing of macromolecules. It appears that the scientific community has not yet realized that supports for these applications are readily available. In addition, monoliths will further substantially advance bioseparations of both small and large molecules in the future.

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Section: Cryogel As Adsorbents For Bioseparation and Bioconversionmentioning

confidence: 99%

Monoliths for fast bioseparation and bioconversion and their applications in biotechnology

Jungbauer¹,

Hahn

2004

J of Separation Science

161

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“…B and T cells were separated by the nylon wool column technique [12], by a discontinuous gra dient of Percoll [13], or by affinity chromatography performed on protein A-Sepharose 6 MB [14], Elu tion of B cells was achieved using isolated mink IgG T cells were identified by their ability to form spontaneous rosettes with goat red blood cells treated with 2-aminoethyl-isothiouronium bromide-hydrobromide (Sigma Chemicals, Munich) [15] or with neuraminidase (Clostridium perfringens type IV, Sigma No. 3001) [ 16].…”

Section: Fractionation and Identification O F Isolated Mncmentioning

confidence: 99%

Aleutian Disease Virus in B and T Lymphocytes from Blood and Spleen and in Bone Marrow Cells from Naturally Infected Mink

Roth¹,

Kaaden²,

Dawen³

et al. 1984

Intervirology

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In the nuclei of 4% of peripheral blood or spleen mononuclear cells (MNC), Aleutian disease virus (ADV)-specific antigens were found by a direct immunofluorescence test. The MNC were further fractionated by nylon wool, affinity chromatography using Staphylococcus aureus protein, or Percoll gradient techniques. ADV and specific antigens were detected in MNC fractions enriched in either the B or T lymphocytes. In the bone marrow, up to 40% antigen-positive cells were demonstrated over a period of 15 months. These findings were confirmed by the detection of infectious virus in the MNC of blood and spleen and in bone marrow cells. Adherent cells from mink and control cells from ADV-negative ferrets were negative in both tests. These findings indicate that ADV exhibits a lymphotropism and can persist in the B- and T-cell fractions from ADV-infected mink over a long period of time. Furthermore, co-cultivation of mink MNC and bone marrow cells with the CCC clone 81 cells was shown to be a reproducible method for the detection of ADV in persistently infected mink.

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“…Antibodies exhibit very specific, high affinity (K d 10 -5 to 10 -10 mol/L) interactions with protein antigens (6); therefore, they are an obvious choice as a biospecific ligand for use in bioselective adsorption technology. Antibodies have been used for the isolation and purification of proteins (7,8), toxins (9), and cells (10)(11)(12). Current developments in monoclonal antibody production (6,(13)(14)(15)(16) and their use in immunoaffinity chromatography (11,17,18) suggests the possibility of using bioselective adsorption technology for the specific removal of B. cereus spores from milk.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of a Bioselective Adsorption Matrix for Removal of Bacillus cereus Spores from Buffer and Milk

Darquea

Swaisgood

Foegeding

1997

LWT - Food Science and Technology

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Separation of cells by affinity chromatography on SpA-sepharose 6MB

Cited by 55 publications

References 7 publications

Monoliths for fast bioseparation and bioconversion and their applications in biotechnology

Monoliths for fast bioseparation and bioconversion and their applications in biotechnology

Aleutian Disease Virus in B and T Lymphocytes from Blood and Spleen and in Bone Marrow Cells from Naturally Infected Mink

Development and Characterization of a Bioselective Adsorption Matrix for Removal of Bacillus cereus Spores from Buffer and Milk

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