Separation of Chlorins and Carotenoids in Capillary Electrophoresis

Belin, Gamze Kavran; Gülaçar, Fazil O.

doi:10.1093/chromsci/45.9.593

Cited by 5 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Much effort has been devoted to developing HPLC-mass spectrometry (HPLC-MS) methods with mainly atmospheric pressure ionization interfaces (APCI) or electrospray ionization interfaces (ESI) [30]. Also, capillary electrophoresis (CE) [31], high 3 performance thin layer chromatography (HPTLC), ultraviolet-visible (UV-Vis), infrared (IR) and Raman spectrometry have been employed as an analytical technique to identify and quantify low levels and various forms of carotenoids [21,[32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Rubashvılı¹,

Tsitsagi²,

Ebralidze³

et al. 2018

Eurasian J Anal Chem

View full text Add to dashboard Cite

The objective of the present study was to develop sequential extraction procedures for the major carotenoids -beta-carotene and lycopene from agro-industrial waste materials -tomato skin, tangerine and orange peels using the ultrasound-assisted extraction and the supercritical fluid extraction techniques. A rapid, effective and selective high performance liquid chromatographic method for quantitative determination of beta-carotene and lycopene in organic extracts solutions was developed and validated with respect to robustness, specificity, linearity-range, accuracy, precision, limit of detection (LOD) and quantitation (LOQ) as well. The effect of the operating pressure, the temperature, the extraction time, the flow rate of supercritical fluid, the sample size, the ultrasound power and the solvent nature used was investigated. The optimal conditions for extraction were found. The LOD and the LOQ are 0.0081µg/mL and 0.00405 µg/mL for beta-carotene, 0.034 µg/mL and 0.0085 µg/mL for lycopene, respectively. No interference was observed. The content of betacarotene per 1 g of dried agro-industrial waste material varies 8.39 -12.75 µg (tomato skin), 25.65 -32.18 µg (tangerine peel), 41.66 -59.16 µg (orange peel) and the content of lycopene -165.11 -179.56 µg (tomato skin), 11.12 -17.91 µg (tangerine peel), 8.37 -10.65 µg (orange peel).

show abstract

Section: Introductionmentioning

confidence: 99%

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Rubashvılı¹,

Tsitsagi²,

Ebralidze³

et al. 2018

Eurasian J Anal Chem

View full text Add to dashboard Cite

show abstract

“…61 Recently capillary electrophoresis (CE) has been found to be a powerful separation technique for the separation of chlorophylls and carotenoids, with advantages such as rapid analysis time, low volume of sample, great separation efficiency, and higher selectivity. 62 Recent advances in CE have been presented. 63 Other available technologies include the use of supercritical uid extraction (SFE) processes.…”

Section: Carotene Quantication and Analysismentioning

confidence: 99%

Carotenoids extraction and quantification: a review

Arvayo-Enríquez¹,

Fernández²,

Gortárez-Moroyoqui³

et al. 2013

Anal. Methods

View full text Add to dashboard Cite

In recent years carotenoids have represented a good alternative for the pharmaceutical and food industries and especially for the human health, they prevent different diseases, such cancer, macular degradation and cataracts. The use of post-harvest residues represents a good alternative for obtaining analytes of interest.The application of enzymatic pretreatment over plant matrices has resulted in an increase in the level of extraction and lowered operating costs. Today, the use of organic solvents such as hexane in this process is frequent, however, new investigations with novel environmentally friendly solvents (non-toxic) promise new cleaner technologies. The aim of this review is to present some methodologies and technologies used today to obtain carotenoids from plant residues, industrial and post-harvest materials.

show abstract

Untargeted metabolite analysis of healthy and Huanglongbing‐infected orange leaves by CE‐DAD

2009

View full text Add to dashboard Cite

Huanglongbing (HLB) is considered the most destructive bacterial citrus disease worldwide. Early detection of HLB is crucial for minimizing its spread. CE was used for the discovery of potential biomarkers for HLB. Optimization of extraction and separation allowed resolving 24 compounds of which 6 were present in significantly higher (p<0.05) concentrations in HLB-infected samples collected monthly for 6 months during the 2007-2008 season. Three of these compounds were identified by mobility and UV spectra as hesperidin, naringenin, and quercetin with mean increase in concentration of 154, 555, and 467%, respectively, above that in healthy leaves. Results support the potential of CE-DAD for untargeted plant metabolomic analysis. CZE, NACE, and MEEKC were compared for metabolic differentiation of healthy and HLB-infected citrus leaves. CZE in a semi-aqueous BGE solution consisting of 8.5 mM of sodium borate (pH 9.3), 15% ACN, and 9% 1-butanol yielded the best peak separation with detection at 190 nm.

show abstract

Separation of Chlorins and Carotenoids in Capillary Electrophoresis

Cited by 5 publications

References 41 publications

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Carotenoids extraction and quantification: a review

Untargeted metabolite analysis of healthy and Huanglongbing‐infected orange leaves by CE‐DAD

Contact Info

Product

Resources

About