Separation of deamidated peptides with mixed-mode chromatography using phospholipid-functionalized monolithic stationary phases

Abstract

Separation of deamidated peptides with mixed-mode chromatography using phospholipid-functionalized monolithic stationary phases.

The benefits of mixed-mode chromatography columns for separation of peptides and protein digests

“…Mixed-mode stationary phases have been mainly used for separation of biologically active molecules, including peptides and proteins [5,[20][21][22][23]. For more complex protein molecules, digestion into smaller fragments/peptides is a necessary step preceding their analysis [1,24].…”

Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography

“…Monolithic materials have attracted much attention and have been well applied in various fields, such as sample pretreatment, chromatographic separation, biomaterials, and simulation of biological processes due to their easy fabrication, wide functional diversity, high permeability, and good chemical stability [ [17] , [18] , [19] , [20] , [21] , [22] ]. For example, some single-chain PC or double-chains PC based monoliths were successfully fabricated for the prediction of drug-membrane interactions [ 23 , 24 ].…”

Macroporous Polymer Monoliths in Thin Layer Format

“…At present, existing flow-through monoliths of various geometries (discs, rods, tubes, and capillary columns) with axial or radial flow have been developed for analytical and industrial applications [23][24][25][26]. A huge number of modern publications on the application of macroporous monoliths are devoted to the various flow-through techniques such as chromatographic separation of small molecules [27,28], synthetic polymers [29], and biological objects (proteins [30], peptides [31], oligosaccharides [32], DNA [33], viruses [34]), solid-phase extraction of wide range of compounds [35][36][37], and flow-through biocatalysis [38][39][40].…”