Separation of desulphoglucosinolates by reversed-phase high-performance liquid chromatography

Michinton, I.; Sang, Joseph P.; Burke, Daniel; Truscott, Roger J.W.

doi:10.1016/s0021-9673(00)84863-3

Cited by 106 publications

(35 citation statements)

References 12 publications

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“…Total nonstructural carbohydrate concentrations were analyzed by the University of Alaska Palmer Laboratory, using the methods of Smith (1969). Isothiocyanate-yielding glucosinolate concentrations were determined at the University of California, Los Angeles by A. Gonzalez and C. Wisdom, using high-performance liquid chromatography (Minchinton et al 1982).…”

Section: Methodsmentioning

confidence: 99%

Patterns of Resource Use by a Drosophilid (Diptera) Leaf Miner on a Native Crucifer

Collinge

Louda

1988

Annals of the Entomological Society of America

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Section: Methodsmentioning

confidence: 99%

Patterns of Resource Use by a Drosophilid (Diptera) Leaf Miner on a Native Crucifer

Collinge

Louda

1988

Annals of the Entomological Society of America

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“…In the meals prepared from older varieties of rapeseed, the dominant glucosinolates were 2-hydroxy-3-but-enyl glucosinolate (2-hydroxy-3-butenyl-GS) but-3-enyl-GS and pent-4-enyl-GS. Methods used to measure these glucosinolates in the past were based on the measurement of their myrosinase hydrolysis products by gas chromatography (GC) and ultra-violet spectrometry (UV) (Appelqvist and Josefsson 1961 ;Wetter and Youngs 1976 (Minchinton et al 1982). Using this technique, two novel indole glucosinolates were isolated and identified (Truscott et al 1982a,b).…”

mentioning

confidence: 99%

Glucosinolate Profiles in the Seed, Root and Leaf Tissue of Cabbage, Mustard, Rapeseed, Radish and Swede

Sang¹,

Minchinton²,

Johnstone³

et al. 1984

Can. J. Plant Sci.

206

122

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“…GLSs were quantified using the internal standard glucotropaeolin and expressed as µmol g -1 dry matter. Peaks were identified with the help of Ruud Verkerk (personal communication) and by comparison with data from Minchinton et al (1982).…”

Section: Gls Measurementsmentioning

confidence: 99%

The Propagation of Variation in Glucosinolate Levels as Effected by Controlled Atmosphere and Temperature in a Broccoli Batch

Schouten¹,

Zhang²,

Tijskens³

et al. 2008

Acta Hortic.

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Broccoli combines high levels of vitamins, fibres and glucosinolates (GLS) with a low calorie count. GLS are precursors for the characteristic broccoli flavour and have anti-carcinogenic properties. This study describes the effect of controlled atmosphere (CA) and temperature on GLS concentrations in broccoli. Data on GLS behaviour and gas exchange were gathered for broccoli heads that were stored at three temperatures and subjected to four levels of O 2 and three levels of CO 2 . The GLS behaviour of three GLS (raphanin, GB and neo-GB) was examined that showed exponential decrease over time, possibly representing the GLS interaction with myrosinase. The most striking feature is the large variation in GLS concentrations at harvest. The propagation of the variation in GLS over time is clearly affected by CA and temperature. Variation in GLS concentrations over time at the same gas conditions and temperature was interpreted with the moment of harvest as main cause of random variation. Assuming that this random variation is normally distributed, the exponential function over time can be transformed into a batch model that describes the changes of variation over time (Schouten et al., 2004, Hertog et al. 2004). The effect of the CA was modelled using the standard gas exchange model. This calibrated gas exchange model was then linked via the reaction rate constant to the batch model to create an integrated batch model. This integrated batch model was subsequently calibrated to describe the variation of the GLS as function of O 2 , CO 2 , time, temperature and the batch parameters (average biological age and standard deviation). The percentage variance accounted for was on average 85%. Considering that this modelling effort is based on destructive GLS measurements, this is a rather high value. All GLS species were retained by suitable (low O 2 , high CO 2 ) gas conditions, but remarkably, raphanin was found to be less affected by temperature, indicating that CA storage and low temperature would both retain this GLS species to the same extent, while low temperature storage is a better option for e.g. neo-GB. INTRODUCTIONBroccoli combines high levels of vitamins, carotenes, fibres, glucosinolates (GLS) and other phytochemicals. Broccoli is a highly perishable vegetable with a shelf-life of only a few days. Colour is the main external quality attribute of broccoli. One of the main internal quality attributes is the level of GLS. The hydrolysis products of GLS are responsible for the characteristic flavour of brassicaceous vegetables and have antimicrobial and cancer-preventing properties. Controlled Atmosphere (CA) is a very effective method to maintain broccoli quality; retention of the green colour is best obtained when 1-2% O 2 combined with 5-10% CO 2 at temperatures between 0 and 5 °C (Jones et al., 2006). However, the effects of CA on the levels of GLS have not been studied. The aim of this paper is to characterise the levels of some GLS of broccoli as affected by CA and temperature. Data on GLS behaviour a...

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Separation of desulphoglucosinolates by reversed-phase high-performance liquid chromatography

Cited by 106 publications

References 12 publications

Patterns of Resource Use by a Drosophilid (Diptera) Leaf Miner on a Native Crucifer

Patterns of Resource Use by a Drosophilid (Diptera) Leaf Miner on a Native Crucifer

Glucosinolate Profiles in the Seed, Root and Leaf Tissue of Cabbage, Mustard, Rapeseed, Radish and Swede

The Propagation of Variation in Glucosinolate Levels as Effected by Controlled Atmosphere and Temperature in a Broccoli Batch

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