Separation of dolichols and polyprenols by straight-phase high-performance liquid chromatography

Keller, R. Kennedy; Rottler, Grant D.; Adair, W. Lee

doi:10.1016/s0021-9673(00)82520-0

Cited by 24 publications

(4 citation statements)

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“…The conventional modern approach usually involves extraction of tobacco samples with organic solvents, separation from leaf pigments by column chromatography, derivatization by methods such as hydrogenation or trimethylsilylation, followed by gas-liquid chromatography (Ellington et al, 1977;Severson et al, 1977;Sheen et al, 1978). Previously gravimetric (Rowland et al, 1956; Woollen et al, 1972) or TLC (Woollen and Jones, 1971) methods were employed; more recently DCI mass spectrometry has been shown to be effective (Einolf, 1984) as well as straight-phase (Keller et al, 1982) and reversed-phase (Smith et al, 1980; Prenzel and Lichtenthaler, 1982) HPLC. A substantial portion of the total solanesol in tobacco is not present as the free alcohol, but as esters of either low molecular weight or fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Direct detection of solanesol in tobacco by proton and carbon-13 magic angle spinning NMR

Wooten¹

1985

J. Agric. Food Chem.

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Section: Introductionmentioning

confidence: 99%

Direct detection of solanesol in tobacco by proton and carbon-13 magic angle spinning NMR

Wooten¹

1985

J. Agric. Food Chem.

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“…(4) for human plasma. A possible explanation for this decrease may be a slowdown of de novo biosynthetic processes with age (10).…”

Section: Resultsmentioning

confidence: 99%

A micromethod for the estimation of blood dolichol

Morris¹,

Pullarkat²

1987

Lipids

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A micromethod for the estimation of dolichol in blood was developed using high performance liquid chromatography. This method can be applied to whole blood or plasma. With detector sensitivity set at 0.005 Absorbance Unit Full Scale, samples as little as 50 microliter are sufficient to carry out the determination. Blood samples from the tail vein of inbred strains of C57 BL/6 NNia-1 mice 3, 6, 12 and 24 mo of age were examined. Blood dolichol levels decreased with age from 163.3 ng/ml at 6 mo to 110.1 ng/ml at 24 mo. The major dolichol homologs were C-85 (10.4%), C-90 (41.6%), C-95 (38.0%) and C-100 (8.1%).

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“…rat liver dolichol kinase showed marked preference for dolichol-16 and dolichol-19 (Keller et al, 1982), although the major constituent of rat liver dolichols is dolichol-18 (Gough & Hemming, 1970).…”

Section: Specificity To Polyprenolmentioning

confidence: 92%

Calmodulin-dependent enzymic phosphorylation of dolichol in Tetrahymena pyriformis

Gandhi¹,

Keenan²

1983

Biochemical Journal

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The Protozoan, Tetrahymena pyriformis, is capable of phosphorylating dolichol in the presence of CTP. Other nucleotides (ATP, UTP and GTP) were ineffective. The enzyme was activated independently by the bivalent cations Mg2+, Mn2+ and Ca2+. The Ca2+ stimulation of the enzyme activity was calmodulin-dependent. A substantial increase in the enzyme activity was seen in the presence of UTP. The apparent Km values for CTP and dolichol, as calculated from the Lineweaver-Burk plot, were 3 mM and 70 microM respectively. Although the presence of detergent was essential, at higher concentrations there was a decrease in the enzyme activity. The enzyme had two pH optima (pH 7.4 and 9.0) and at both the activity was Ca2+-calmodulin-dependent.

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Separation of dolichols and polyprenols by straight-phase high-performance liquid chromatography

Cited by 24 publications

References 4 publications

Direct detection of solanesol in tobacco by proton and carbon-13 magic angle spinning NMR

Direct detection of solanesol in tobacco by proton and carbon-13 magic angle spinning NMR

A micromethod for the estimation of blood dolichol

Calmodulin-dependent enzymic phosphorylation of dolichol in Tetrahymena pyriformis

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