Separation of Major Mononitro-aromatic Compounds by Reversed-phase High-performance Liquid Chromatography.

Abstract

A simple analytical method was developed to determine p-nitrochlorobenzene (p-NCB) in working environmental air by reversed-phase high-performance liquid chromatography (HPLC). Nitrobenzene, nitrotoluene isomers, and NCB isomers were separated and p-NCB was determined by reversed-phase HPLC as follows: column; Hitachi gel #3056 (4.0 mm I.D. x 150 mm), mobile phase; 2-propanol: water (40:60, v/ v) with a flow rate of 0.8 ml/min, column temperature; 50°C, detector; UV detector at 270 nm. Nitrobenzene, o-, p-nitrotoluene, and o-, m-NCB were separated from p-NCB, but m-nitrotoluene could not be completely separated from p-NCB. The limit of detection (signal-to-noise ratio of 3:1) of p-NCB was 0.3 ng. Repeatability of the HPLC method was excellent. The HPLC method enabled to determine one-tenth of the administrative level (1 mg/m3) of p-NCB, since the lower limit of determination was 0.05 mg/m3. Therefore, the present HPLC method proved to be applicable to the determination of p. NCB in working environmental air.

Highly Efficient Multiple-Anchored Fluorescent Probe for the Detection of Aniline Vapor Based on Synergistic Effect: Chemical Reaction and PET

“…Over the past decades, environmental issues are becoming more and more serious and have drawn a great deal of attention. Among environmental pollutants, the detection of aniline has gained extra attention because aniline as an intermediate of many raw and fine chemicals is widely used in industrial processes, which not only leads to heavy water and air pollution , but also induces severe human diseases. − Up to now, a series of analytical methods such as high performance liquid chromatography (HPLC), , electrochemistry, , GC-MS, , and spectrofluorimetry − have been developed to detect aniline in water. However, these methods cannot meet needs for the onsite and real-time determination of aniline vapor in air with selectivity and sensitivity.…”