Separation of Membrane-Bound Compounds by Solid-Supported Bilayer Electrophoresis

Daniel, Susan; Diaz, Arnaldo J.; Martinez, Kelly M.; Bench, Bennie J.; Albertorio, Fernando; Cremer, Paul S.

doi:10.1021/ja0720816

Cited by 62 publications

(83 citation statements)

References 22 publications

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“…This lipid-dye conjugate is usually synthesized by reacting the amine of a phosphatidylethanolamine lipid with the lower aromatic ring of Texas Red sulfonyl chloride, which resides at either the ortho-or para-positions, [ 48 ] resulting in an isomeric mixture. Interestingly, the fl uorescence intensity of the ortho isomer has been shown to be pH dependent, [ 49 ] with lower intensities under basic conditions.…”

Section: Fluorescence-based Analysismentioning

confidence: 99%

Photoresponsive Cucurbit[8]uril‐Mediated Adhesion of Bacteria on Supported Lipid Bilayers

Sankaran

Weerd

Voskuhl

et al. 2015

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In this work, the development of a photoresponsive platform for the presentation of bioactive ligands to study receptor-ligand interactions has been described. For this purpose, supramolecular host-guest chemistry and supported lipid bilayers (SLBs) have been combined in a microfluidic device. Quartz crystal microbalance with dissipation monitoring (QCM-D) studies on methyl viologen (MV)-functionalized oligo ethylene glycol-based self-assembled monolayers, gel and liquid-state SLBs have been compared for their nonfouling properties in the case of ConA and bacteria. In combination with bacterial adhesion test, negligible nonspecific bacterial adhesion is observed only in the case of methyl-viologen-modified liquid-state SLBs. Therefore, liquid-state SLBs have been identified as most suitable for studying specific cell interactions when MV is incorporated as a guest on the surface. The photoswitchable supramolecular ternary complex is formed by assembling cucurbit[8]uril (CB[8]) and an azobenzene-mannose conjugate (Azo-Man) onto MV-functionalized liquid-state SLBs and the assembly process has been characterized using QCM-D and fluorescence techniques. Mannose has been found to enable binding of E. coli via cell-surface receptors on the nonfouling supramolecular SLBs. Optical switching of the azobenzene moiety allows us to "erase" the bioactive surface after bacterial binding, providing the potential to develop reusable sensors. Localized photorelease of bacterial cells has also been shown indicating the possibility of optically guiding cellular growth, migration, and intercellular interactions.

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Section: Fluorescence-based Analysismentioning

confidence: 99%

Photoresponsive Cucurbit[8]uril‐Mediated Adhesion of Bacteria on Supported Lipid Bilayers

Sankaran

Weerd

Voskuhl

et al. 2015

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“…However, most reported methods are based on electrophoresis, 21 which imposes the crucial restriction that uncharged molecules cannot be manipulated in ultrasmall spaces. To overcome this limitation, self-spreading lipid bilayers have recently attracted interest because of their superior molecular transport ability ( Fig.…”

Section: Manipulation Of a Small Number Of Molecules In Self-mentioning

confidence: 99%

Molecule Manipulation at Electrified Interfaces using Metal Nanogates

et al. 2014

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Previously documented results on molecule manipulation using metal nanogates are comprehensively reviewed to conclude novel characteristics, which could be widely used for the developments of the systems at electrified interfaces. Novel systems to segregate molecules from self-spreading lipid bilayers in electrolyte solutions have been developed by using metal nanogate structures on solid surfaces. The spatial distribution of target molecules in the lipid bilayer changes depending on the molecule characteristics, such as charge, size, and flexibility. Energy dissipation during the spreading of the molecules on the surface contributes to the compression of the lipid bilayer at the nanogate, leading to the formation of a gradient of the electrochemical potential of the bilayer system including the target molecule in the vicinity of the nanogate (<100 nm from the gate). The gradient results in a segregation force being applied to target molecules in the order of 10 −15 N per molecule. The segregation property can be tuned by changing the electrolytes, lipid molecules, temperature, and the width and surface hydrophobicity of the metal nanogate. Introduction of asymmetry to the nanogate leads to further effective diffusion control in a direction perpendicular to the spreading. It has been demonstrated that target molecules with a different number of glycolipid per protein in the lipid bilayer are effectively separated based on the Brownian ratchet mechanism.

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“…We used water-soluble cholesterol to demonstrate the effects of cholesterol, which is able to pass through the plasma membrane due to the presence of methyl-ß-cyclodextrin (Daniel et al, 2004(Daniel et al, , 2007, and it is a common approach to load cells with cholesterol instead of using lipoproteins. We used a dose at which no toxic effects were found in our cultures in pilot experiments.…”

Section: Cholesterol Stimulates Estradiol Synthesis In Hippocampal Cumentioning

confidence: 99%

Cholesterol‐promoted synaptogenesis requires the conversion of cholesterol to estradiol in the hippocampus

et al. 2009

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Cholesterol of glial origin promotes synaptogenesis (Mauch et al., (2001) Science 294:1354-1357). Because in the hippocampus local estradiol synthesis is essential for synaptogenesis, we addressed the question of whether cholesterol-promoted synapse formation results from the function of cholesterol as a precursor of estradiol synthesis in this brain area. To this end, we treated hippocampal cultures with cholesterol, estradiol, or with letrozole, a potent aromatase inhibitor. Cholesterol increased neuronal estradiol release into the medium, the number of spine synapses in hippocampal slice cultures, and immunoreactivity of synaptic proteins in dispersed cultures. Simultaneous application of cholesterol and letrozole or blockade of estrogen receptors by ICI 182 780 abolished cholesterol-induced synapse formation. As a further approach, we inhibited the access of cholesterol to the first enzyme of steroidogenesis by knock-down of steroidogenic acute regulatory protein, the rate-limiting step in steroidogenesis. A rescue of reduced synaptic protein expression in transfected cells was achieved by estradiol but not by cholesterol. Our data indicate that in the hippocampus cholesterol-promoted synapse formation requires the conversion of cholesterol to estradiol.

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Separation of Membrane-Bound Compounds by Solid-Supported Bilayer Electrophoresis

Cited by 62 publications

References 22 publications

Photoresponsive Cucurbit[8]uril‐Mediated Adhesion of Bacteria on Supported Lipid Bilayers

Photoresponsive Cucurbit[8]uril‐Mediated Adhesion of Bacteria on Supported Lipid Bilayers

Molecule Manipulation at Electrified Interfaces using Metal Nanogates

Cholesterol‐promoted synaptogenesis requires the conversion of cholesterol to estradiol in the hippocampus

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