Separation of mesenchymal stem cells with magnetic nanosorbents carrying CD105 and CD73 antibodies in flow-through and batch systems

Odabaş, Sedat; Sayar, Filiz; Güven, Güldem; Yanıkkaya-Demirel, G.; Pi̇şki̇n, Erhan

doi:10.1016/j.jchromb.2007.11.017

Cited by 30 publications

(24 citation statements)

References 26 publications

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“…In contrast to hBMMSCs, both hDPSCs and cDPSCs did not express a considerable level of CD105. However, some of the previous studies have reported CD105 positivity in hDPSCs similar to hBMMSCs (11,31).…”

Section: Discussionmentioning

confidence: 86%

Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars

Dissanayaka

Zhu

Zhang

et al. 2011

Journal of Endodontics

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Section: Discussionmentioning

confidence: 86%

Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars

Dissanayaka

Zhu

Zhang

et al. 2011

Journal of Endodontics

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“…In addition, these markers have been used to isolate and clonally expand enriched mesenchymal stem cell cultures (30). Thus, we evaluated the expression of the CD105 and CD73 genes as a dependent measure of the relative presence of stem cells.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Delivery of Mesenchymal Stem Cells into the Root Canal Space of Necrotic Immature Teeth after Clinical Regenerative Endodontic Procedure

Lovelace

Henry

Hargreaves

et al. 2011

Journal of Endodontics

416

255

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“…Flow cytometric analysis of these cells was used to assess the purity of the hMSC cultures. Cells positive for CD73 and CD105 were judged to be hMSCs [23–26]. There was no detectable contamination with hematopoietic cells as judged by the absence of CD34 [27] and CD45 [28], which are markers of the hematopoietic lineage (Fig.…”

Section: Methodsmentioning

confidence: 99%

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

et al. 2013

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Shockwave fractures treatment promotes bone healing of nonunion fractures. In this study, we investigated whether this effect could be due to adenosine 5’-triphosphate (ATP) release-induced differentiation of human mesenchymal stem cells (hMSCs) into osteoprogenitor cells. Cultured bone marrow-derived hMSCs were subjected to shockwave treatment and ATP release was assessed. Osteogenic differentiation and mineralization of hMSCs were evaluated by examining alkaline phosphatase activity, osteocalcin production, and calcium nodule formation. Expression of P2X7 receptors and c-fos and c-jun mRNA was determined with real-time reverse transcription polymerase chain reaction and Western blotting. P2X7-siRNA, apyrase, P2 receptor antagonists, and p38 MAPK inhibitors were used to evaluate the roles of ATP release, P2X7 receptors, and p38 MAPK sig naling in shockwave-induced osteogenic hMSCs differentiation. Shockwave treatment released significant amounts (~7 μM) of ATP from hMSCs. Shockwaves and exogenous ATP induced c-fos and c-jun mRNA transcription, p38 MAPK activation, and hMSC differentiation. Removal of ATP with apyrase, targeting of P2X7 receptors with P2X7-siRNA or selective antagonists, or blockade of p38 MAPK with SB203580 prevented osteogenic differentiation of hMSCs. Our findings indicate that shockwaves release cellular ATP that activates P2X7 receptors and downstream signaling events that caused osteogenic differentiation of hMSCs. We conclude that shockwave therapy promotes bone healing through P2X7 receptor signaling, which contributes to hMSC differentiation.

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Separation of mesenchymal stem cells with magnetic nanosorbents carrying CD105 and CD73 antibodies in flow-through and batch systems

Cited by 30 publications

References 26 publications

Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars

Characterization of Dental Pulp Stem Cells Isolated from Canine Premolars

Evaluation of the Delivery of Mesenchymal Stem Cells into the Root Canal Space of Necrotic Immature Teeth after Clinical Regenerative Endodontic Procedure

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors

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