Separation of mistletoe lectins based on the degree of glycosylation using boronate affinity chromatography

Glycation of antibodies: Modification, methods and potential effects on biological functions

“…The glycosylation species also interact with the BAC surface, which may cause some non-specific binding. 46,47 The quantitation is also an estimate because it cannot differentiate between proteins with a single glycation or multiple glycations.…”

Selective extraction and enrichment of glycoproteins based on boronate affinity SPME and determination by CIEF-WCID

“…Due to these merits, boronic-acid-functionalized materials have increasingly attracted attention in recent years [16][17][18]. They have been used in boronate affinity chromatography (BAC) [19] and boronate affinity monolithic column [20], and have shown high affinity and extraordinarily specific recognition ability towards cis-diol-containing biomolecules.…”

Beyond the Sequon: Sites of N-Glycosylation

“…The physical properties of carbohydrates that distinguish them from protein can be used to enrich glycopeptides and glycoproteins. Typical enrichment strategies based on the physical properties of glycans include hydrophilic interaction chromatography (Mysling et al, 2010, Gilar et al, 2011, Christiansen et al, 2010, phenyl boronic acid (Li et al, 2000, Li et al, 2001) and hydrazide (Zhang & Aebersold, 2006, Zhang et al, 2003 attachment. A key mechanism mediating the functional roles of glycans in many biological systems is recognition of specific glycan structures by proteins, or lectins.…”