Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection

Karlsson, Göran; Winge, Stefan; Sandberg, Helena

doi:10.1016/j.chroma.2005.08.025

Cited by 124 publications

(62 citation statements)

References 18 publications

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“…The most popular direct detection modes are refractive index, evaporative light scattering [14][15][16], charged aerosol [17], and when high sensitivity is needed, pulse electrochemical detection [18,19]. More selective and sensitive detection can also be reached by fluorescence after derivatization with a fluorophore (e.g.…”

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confidence: 99%

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

et al. 2015

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Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV-detectionThis work focuses on the development of a CE method allowing, for the first time, the simultaneous separation of the underivatized first seven cellodextrin oligomers (glucose, cellobiose, cellotriose, cellotetraose, cellopentaose, cellohexaose, and celloheptaose), with a view to analyze the hydrolysates obtained after partial acid depolymerization of nitrocellulose, and eight carbohydrates (ribose, xylose, fructose, mannose, galactose, maltose, lactose, and sucrose), which might be potential interfering compounds in explosives samples. Separation was achieved with a highly alkaline BGE containing sodium chloride and direct mid-UV-absorbance detection was performed after photo-oxidation in the detection window. EOF was reversed to speed up the analysis using a dynamic capillary coating by hexadimethrine bromide. A central composite design was carried out to determine the effects of BGE conductivity and sodium hydroxide concentration on resolutions between neighboring peaks, and analysis time. A desirability analysis on modeled responses was applied to maximize resolutions and to minimize analysis time. The simultaneous analysis in 20 min total runtime of the 15 carbohydrates plus internal reference (naphthalene sulfonate) was carried out at 25°C with a BGE composed of 77.4 mM NaOH and 183 mM NaCl to adjust the conductivity at the optimum value. Finally, the resolution robustness was checked. This new method should also be of interest to monitor food and nonfood crop products. Keywords:Cellodextrins / Capillary electrophoresis / Design of experiments / Direct UV detection / Oligosaccharide DOI 10.1002/elps.201400605Additional supporting information may be found in the online version of this article at the publisher's web-site IntroductionCarbohydrates are ubiquitous compounds in the living world, as natural and degradation products, metabolites, or conjugates. They are also widely used as food or beverage additives. This class of compounds of tremendous complexity actually encompasses a variety of molecules differing in size (mono-, oligo-, and polysaccharides), epimerization or isomerization, glycosidic linkage, branching, and functionality , and mixed modes [11,13] have all been commonly employed. Separations have been based on hydrogen bonding, size, complexing ability, and in some cases charge. As far as detection is concerned, great difficulties are encountered due to the lack of a chromophoric moiety. According to the sensitivity requirements and separation mode, different detection strategies have been implemented, namely, without-or with pre-or postcolumn

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Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

et al. 2015

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“…As an alternative approach to reversed-phase liquid chromatography (RPLC) which is an indispensable and versatile technique for the separation of a wide range of analytes, hydrophilic interaction chromatography (HILIC) plays an important role in separating highly polar and hydrophilic compounds [1][2][3][4][5][6][7][8][9][10], such as oligosaccharides, peptides, nucleosides, polar pharmaceuticals, etc. Substantial separation materials have been designed specially for HILIC [8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase

Shen

Guo

Cai

et al. 2012

Journal of Chromatography A

105

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“…Similar column eluted with 82% acetonitrile and ammonium formate adjusted to pH 5.5 with formic acid was used for separation of six monosaccharides detected by evaporative light scattering detection (ELSD) [23]. Authors investigated the influence of ammonium formate concentration and temperature on retention and separation selectivity.…”

Section: Amide Stationary Phasesmentioning

confidence: 99%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

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Summary. Hydrophilic interaction chromatography (HILIC) is a liquid chromatographytechnique when a polar stationary phases -silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC -the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC -faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.

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Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection

Cited by 124 publications

References 18 publications

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

Analysis of underivatized cellodextrin oligosaccharides by capillary electrophoresis with direct photochemically induced UV‐detection

Preparation and chromatographic evaluation of a cysteine-bonded zwitterionic hydrophilic interaction liquid chromatography stationary phase

Application of hydrophilic interaction chromatography in phytochemical analysis

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