Separation of nano- and micro-sized materials by hyphenated flow and centrifugal field-flow fractionation

Kato, Haruhisa; Nakamura, Ayako

doi:10.1039/c3ay42203h

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…The essential requirement for adverse-FFF is implementing two opposing forces, which scale differently as a function of solute size and have controllable field strengths. One such device would be the separation system reported by Kato and Nakamura [58]. Although current operation is reportedly hyphenated, this system could conceivably be modified to operate in adverse-mode.…”

Section: Implementation and Feasibilitymentioning

confidence: 99%

“…where P is the percent difference of the solute to the carrier fluid density. The percent difference P varies widely, but let us say P ≈ 5% as appropriate for polystyrene [58] or polydimethylsiloxane [52]. Although N G = 1 for gravitational-FFF, it can be many thousands for centrifugal-FFF (say ∼10,000 [43]).…”

Section: Implementation and Feasibilitymentioning

confidence: 99%

“…• For electrical-FFF, Λ 1 ∼ −10 −7 V/V eff , where V eff ∼ 0.1 V is the effective voltage between plates [75] and an electrophoretic mobility ∼10 −4 cm 2 V −1 s −1 is assumed [56]. • For flow-FFF, Λ 1 ∼ −10 −7 s −1 V 0 /V C , where the channel volume is V 0 ∼ 10 −6 m 3 and the cross-flow rateV C ∼ 1 mL/min [58].…”

Section: Implementation and Feasibilitymentioning

confidence: 99%

“…The prescribed and simultaneous application of two aligned external fields is termed additive-mode FFF [56]. Likewise, Kato and Nakamura have successfully developed a hyphenated flow-FFF and centrifugal-FFF apparatus, in which the two fields are applied in series [58].…”

Section: Introductionmentioning

confidence: 99%

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Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Shendruk¹,

Slater

2015

Chromatography

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A novel field-flow fractionation (FFF) technique, in which two opposing external forces act on the solute particles, is proposed. When the two external forces are sufficiently strong and scale differently as a function of the solutes' property of interest (such as the solute particle size), a sharp peak in the retention ratio (dramatic drop in elution time) is predicted to exist. Because the external forces oppose one another, we refer to this novel technique as adverse-mode FFF. The location of this peak is theoretically predicted and its ideal width estimated. The peak can become quite sharp by simultaneously increasing the strength of both fields, suggesting that adverse-mode FFF could be a useful technique for accurately measuring single species solute size.

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Section: Implementation and Feasibilitymentioning

confidence: 99%

Section: Implementation and Feasibilitymentioning

confidence: 99%

Section: Implementation and Feasibilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Shendruk¹,

Slater

2015

Chromatography

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“…31 In fact, many researchers capitalize on the high rotational frequency to provide particle sedimentation, fractionation, isolation, separation among others. 22,[32][33][34][35][36] Recent advances capitalize on microfluidic design to achieve passive valving and inertial focusing within the centrifugal platform. 37,38 Carboxylated microbeads are also recently utilized for specific biomolecular capture and extraction in a centrifugal platform.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic size separation of cells and particles using a swinging bucket centrifuge

2015

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Biomolecular separation is crucial for downstream analysis. Separation technique mainly relies on centrifugal sedimentation. However, minuscule sample volume separation and extraction is difficult with conventional centrifuge. Furthermore, conventional centrifuge requires density gradient centrifugation which is laborious and time-consuming. To overcome this challenge, we present a novel size-selective bioparticles separation microfluidic chip on a swinging bucket minifuge. Size separation is achieved using passive pressure driven centrifugal fluid flows coupled with centrifugal force acting on the particles within the microfluidic chip. By adopting centrifugal microfluidics on a swinging bucket rotor, we achieved over 95% efficiency in separating mixed 20 μm and 2 μm colloidal dispersions from its liquid medium. Furthermore, by manipulating the hydrodynamic resistance, we performed size separation of mixed microbeads, achieving size efficiency of up to 90%. To further validate our device utility, we loaded spiked whole blood with MCF-7 cells into our microfluidic device and subjected it to centrifugal force for a mere duration of 10 s, thereby achieving a separation efficiency of over 75%. Overall, our centrifugal microfluidic device enables extremely rapid and label-free enrichment of different sized cells and particles with high efficiency.

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Field‐flow fractionation for nanoparticle characterization

Lespès

Pont

2021

J of Separation Science

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This review presents field-flow fractionation: The elements of theory enable the link between the retention and the characteristics of the nanometer-sized analytes to be highlighted. In particular, the nature of force and its way of being applied are discussed. Four types of forces which determine four types of techniques were considered: hydrodynamic, sedimentation, thermal, and electrical; this is to show the importance of the choice of technique in relation to the characterization objectives. Then the separation performance is presented and compared with other separation techniques: field-flow fractionation has the greatest intrinsic separation capability. The characterization strategies are presented and discussed; on the one hand with respect to the characteristics needed for the description of nanoparticles; on the other hand in connection with the choice of the nature of the force, and also of the detectors used, online or offline. The discussion is based on a selection of published study examples. Finally, current needs and challenges are addressed, and as response, trends and possible characterization solutions.

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Separation of nano- and micro-sized materials by hyphenated flow and centrifugal field-flow fractionation

Cited by 6 publications

References 19 publications

Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Adverse-Mode FFF: Multi-Force Ideal Retention Theory

Microfluidic size separation of cells and particles using a swinging bucket centrifuge

Field‐flow fractionation for nanoparticle characterization

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