Separation of natural product using columns packed with Fused‐Core particles

Abstract

Three HPLC columns packed with 3 microm, sub-2 microm, and 2.7 microm Fused-Core (superficially porous) particles were compared in separation performance using two natural product mixtures containing 15 structurally related components. The Ascentis Express C18 column packed with Fused-Core particles showed an 18% increase in column efficiency (theoretical plates), a 76% increase in plate number per meter, a 65% enhancement in separation speed and a 19% increase in back pressure compared to the Atlantis T3 C18 column packed with 3 microm particles. Column lot-to-lot variability for critical pairs in the natural product mixture was observed with both columns, with the Atlantis T3 column exhibiting a higher degree of variability. The Ascentis Express column was also compared with the Acquity BEH column packed with sub-2 microm particles. Although the peak efficiencies obtained by the Ascentis Express column were only about 74% of those obtained by the Acquity BEH column, the 50% lower back pressure and comparable separation speed allowed high-efficiency and high-speed separation to be performed using conventional HPLC instrumentation.

Fast and simultaneous determination of phenolic compounds and caffeine in teas, mate, instant coffee, soft drink and energetic drink by high-performance liquid chromatography using a fused-core column

“…However, only a few real applications of this new column technology for the determination of phytochemicals in natural products have been reported [24][25][26][27][28]. Most of these applications are limited to a few compound classes in one sample type but indicate enormous potential to reduce analysis times as well as to improve separation of complex mixtures.…”

Identification of irradiated meats by determining o- and m-tyrosine as markers

“…Separation was tried with fluorescence detection at its characteristic wavelength, λex 275 nm and λem 305 nm by using various combinations of methanol, acetonitrile and additives like formic acid on these columns to obtain adequate response, good peak shape and short analysis time. Atlantis T3 column has been previously reported in several papers for superior separation and peak shape for polar substances (Heinig & Bucheli, 2008;Xiang et al, 2010;Yang, Litwinski, Pursch, McCabe, & Kuppannan, 2009). In our study, the T3 column was also used in order to achieve complete separation of p-, m-and o-tyrosine from interfering co-eluents.…”

Emerging Analytical Separation Techniques with High Throughput Potential for Pharmaceutical Analysis, Part I: Stationary Phase and Instrumental Developments in LC

“…As they are relatively easy and inexpensive to change, simple modifications of conventional HPLC instruments may satisfy to perform very efficient analyses with fused-core particulate columns. In comparison with totally porous sub-2 m particles, recent studies in isocratic and gradient mode showed that fused-core particles could provide high-speed separations with comparable or slightly compromised performances at modest backpressures [66][67][68][69][70]. Fig.…”