Separation of nonsteroidal anti-inflammatory drugs by capillary electrophoresis using nonaqueous electrolytes

Abstract

The aim of the present work was to investigate the separation of nonsteroidal anti‐inflammatory drugs (NSAIDs: niflumic acid, flufenamic acid, piroxicam, alclofenac, tiaprofenic acid, flurbiprofen, suprofen, ketoprofen, naproxen, indomethacin, carprofen, indoprofen, sulindac) in capillary electrophoresis (CE) using completely nonaqueous systems. The influence of different parameters such as nature and proportion of organic solvent (methanol, acetonitrile, 2‐propanol), apparent pH (ranging from 7 to 9) and temperature (ranging from 25 to 40oC) on selectivity and migration times were studied systematically in an uncoated fused‐silica capillary. A nonaqueous electrolyte made of 50 mM ammonium acetate — 13.75 mM ammonia in methanol proved to resolve 11 NSAIDs at 25oC and 13 NSAIDs at 36oC, both within 13 min and without a modifier besides the methanol itself. The same buffer containing 30% acetonitrile provides a satisfactory separation for 13 NSAIDs within 14 min at 25oC.

Partition coefficients of organic compounds between water and imidazolium-, pyridinium-, and phosphonium-based ionic liquids

“…4 ]) and water at room temperature. We also used the SPM to study these biphasic systems to both predict partitioning and to chemically interpret the LSER coefficients [29] f Data from reference [30] g Data from reference [32] h Data from reference [33] obtained. Requirements to use the SPM are clearly described in the review of Vitha and Carr [19], which were summarized in our previous work.…”

Analyses of Non-steroidal Anti-inflammatory Drugs in Environmental Water Samples with Microemulsion Electrokinetic Chromatography

“…2,3,[6][7][8][9][10][11][12][13][14] Microemulsion electrokinetic chromatography (MEEKC), which has a similar separation mechanism as micellar electrokinetic chromatography (MEKC), has been demonstrated to be a reliable separation technique for a wide range of analytes, including NSAIDs compounds. [15][16][17][18][19][20][21] For all CE derived techniques, including MEKC and MEEKC, on-capillary UV detection is still employed as the major detection mode.…”

Determination of indomethacin in plasma by micellar electrokinetic chromatography with UV detection for premature infants with patent ducts arteriosus

“…Numerous methods are available for the determination of indomethacin, including gas chromatography (GC) [4,5], GC-mass spectrometry [6], high performance liquid chromatographic (HPLC) methods [7][8][9][10][11][12][13][14][15][16][17][18], HPLC-mass spectrometry [19,20] and capillary electrophoresis (CE) [21][22][23][24][25]. Indomethacin has an active carboxyl group in the structure.…”