Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry

Moravcová, Dana; Haapala, Markus; Planeta, Josef; Hyötyläinen, Tuulia; Kostiainen, Risto; Wiedmer, Susanne Κ.

doi:10.1016/j.chroma.2014.11.015

Cited by 35 publications

(17 citation statements)

References 38 publications

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“…Chromatographic separations of extremely hydrophilic and negatively charged nucleotides were especially challenging, due to the adsorptive properties of phosphorylated species [18]. According to the previous reports, the HILIC column was suitable for separation of these polar compounds, especially for high polar nucleotides [11,19].…”

Section: Optimization Of Hilic-uhplcmentioning

confidence: 98%

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Zhou

Wang

et al. 2015

J of Separation Science

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A rapid and sensitive hydrophilic interaction ultra high performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry method was validated for the simultaneous determination of 20 nucleobases, nucleosides, and nucleotides (within 3.5 min), and then was employed to test the functional food of Luo-Han-Guo samples. The analysis showed that the Luo-Han-Guo was rich in guanosine and uridine, but contained trace levels of the other target compounds. Chemometrics methods were employed to identify 40 batches of Luo-Han-Guo samples from different cultivated forms, regions and varieties. Unsupervised hierarchical cluster analysis and principal component analysis were used to classify Luo-Han-Guo samples based on the level of the 20 target compounds, and the supervised learning method of counter propagation artificial neural network was utilized to further separate clusters and validate the established model. As a result, the samples could be clustered into three primary groups, in which correlation with cultivated varieties was observed. The present strategy could be applied to the investigation of other edible plants containing nucleobases, nucleosides, or nucleotides.

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Section: Optimization Of Hilic-uhplcmentioning

confidence: 98%

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Zhou

Wang

et al. 2015

J of Separation Science

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“…Separation of the extremely hydrophilic and negatively charged nucleotides is a challenge in either LC or RP‐CEC . HI‐CEC may provide a solution to this issue.…”

Section: Resultsmentioning

confidence: 99%

Preparation of phosphorylcholine‐based hydrophilic monolithic column and application for analysis of drug‐related impurities with capillary electrochromatography

Qiu

Zhang

et al. 2016

Electrophoresis

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A hydrophilic monolithic CEC column was prepared by thermal copolymerization of zwitterionic monomer 2-methacryloyloxyethyl phosphorylcholine (MPC), pentaerythritol triacrylate (PETA), either methacrylatoethyl trimethyl ammonium chloride (META) or sodium 2-methylpropene-1-sulfonate (MPS) in a polar binary porogen consisting of methanol and THF. A typical hydrophilic interaction LC retention mechanism was observed for low-molecular weight polar compounds including amides, nucleotides, and nucleosides in the separation mode of hydrophilic interaction CEC, when high content of ACN (>60%) was used as the mobile phase. The effect of the electrostatic interaction between the analytes and the stationary phase was found to be negligible. The poly(MPC-co-PETA-co-META or MPS) monolithic columns have an average column efficiency of 40 000 plates/m and displayed with a satisfactory repeatability in terms of migration time and peak areas. Finally, the column was successfully applied to determine the impurities of a positively charged drug pramipexole which are often separated by ion pair RP chromatography due to their high hydrophilicity. All four components can be baseline separated within 5 min with BGE consisting of ACN/20 mM ammonium formate buffer (pH 3.0; 80/20).

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“…Nucleosides and benzoic acid derivatives, which are commonly used to evaluate the selectivity of HILIC stationary phases, 24,25 were chosen as the model compounds to investigate the chromatographic properties of the poly(VBSIm-AM-MBA) monolithic column. The log P and pK a values of the elected nucleosides and benzoic acid derivatives are shown in Table 2.…”

Section: Chromatographic Evaluation Of the Poly(vbsim-am-mba) Monolitmentioning

confidence: 99%

Ionic liquid-based zwitterionic organic polymer monolithic column for capillary hydrophilic interaction chromatography

Wang

Chen

et al. 2015

Analyst

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In the current study, a novel ionic liquid-based zwitterionic organic polymer monolithic column was developed by copolymerizing 1-vinyl-3-(butyl-4-sulfonate) imidazolium, acrylamide and N,N'-methylenebisacrylamide in a quaternary porogenic solvent consisting of formamide, dimethyl sulphoxide, polyethylene glycol 8000 and polyethylene glycol 10,000 for capillary hydrophilic interaction chromatography. The monolithic stationary phase was optimized by adjusting the amount of monomer in the polymerization solution along with the composition of porogenic solvent. The optimized monolith exhibited excellent selectivity and favorable retention for nucleosides and benzoic acid derivatives. The primary factors affecting the separation efficiency of the monolithic column (including acetonitrile content, pH, and buffer salt concentration in the mobile phase) have been thoroughly evaluated. Excellent reproducibility of the retention times for five nucleosides was achieved, with relative standard deviations of run-to-run (n = 3), column-to-column (n = 3) and batch-to-batch (n = 3) in the range of 0.18-0.48%, 2.33-4.20% and 3.07-6.50%, respectively.

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Separation of nucleobases, nucleosides, and nucleotides using two zwitterionic silica-based monolithic capillary columns coupled with tandem mass spectrometry

Cited by 35 publications

References 38 publications

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Preparation of phosphorylcholine‐based hydrophilic monolithic column and application for analysis of drug‐related impurities with capillary electrochromatography

Ionic liquid-based zwitterionic organic polymer monolithic column for capillary hydrophilic interaction chromatography

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