Separation of particles in nonaqueous suspensions by thermal-electrical field-flow fractionation

Liu, Guangyue; Giddings, J. Calvin

doi:10.1021/ac00003a021

Cited by 38 publications

(15 citation statements)

References 39 publications

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“…Also, experiments on hyphenated electrical-Thermal FFF were carried out (Liu and Giddings, 1991) where both transverse electric field and temperature gradient were used. These experiments allow us to evaluate the electrophoretic mobilities of polymer particles that are the objects of such experiments (polystyrene latex in acetonitrile) as b a % 10 À7 -10 À6 cm 2 V À1 s À1 .…”

Section: Discussionmentioning

confidence: 99%

Hyphenated thermal field flow fractionation–capillary electrophoresis

Semenov

1998

J. Mol. Recognit.

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The possibility was considered to use the transverse thermophoresis of analytes in the capillary for capillary electrophoresis (CE) to control the separation process, decrease the peak width due to thermal effects and provide new separation parameters in CE. As the examination has shown, in non-aqueous buffers the Joule heating in the capillary for CE can provide transverse temperature gradients comparable with the temperature gradients in conventional devices for thermal field flow Fractionation (ThFFF). It was proposed to use the non-uniform velocity profile of analytes caused by the transverse temperature gradient and the temperature dependence of the buffer viscosity for the FFF-like separation of analytes besides CE separation. The expressions for the peak parameters have been derived, where the non-uniform transverse analyte concentration distribution due to the thermophoresis is taken into account, and the possibilities based on FFF-CE principles are discussed. As possible objects of this hyphenated technique, macromolecules and particles are considered.

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Section: Discussionmentioning

confidence: 99%

Hyphenated thermal field flow fractionation–capillary electrophoresis

Semenov

1998

J. Mol. Recognit.

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“…ThFFF has been used mainly for separations of organosoluble polymers [30] and to a lesser extent separations of particles [51,52]. The "field" used in ThFFF is a temperature gradient (Fig.…”

Section: Thfffmentioning

confidence: 99%

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

Williams¹,

Lee

2006

J of Separation Science

125

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This review summarizes developments and applications of flow and thermal field-flow fractionation (FFF) in the areas of macromolecules and supramolecular assemblies. In the past 10 years, the use of these FFF techniques has extended beyond determining diffusion coefficients, hydrodynamic diameters, and molecular weights of standards. Complex samples as diverse as polysaccharides, prion particles, and block copolymers have been characterized and processes such as aggregation, stability, and infectivity have been monitored. The open channel design used in FFF makes it a gentle separation technique for high- and ultrahigh-molecular weight macromolecules, aggregates, and self-assembled complexes. Coupling FFF with other techniques such as multiangle light scattering and MS provides additional invaluable information about conformation, branching, and identity.

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“…With regard to Eqs. (3,4,7,8), and (9), the precision of the d p , determined from the experimental V R or t R data with the use of a calibration function (Eq. 8 or 9), will be given by the repeatability of the V R or t R and influenced by the stability of the main experimental variable, DT.…”

Section: Micro-thermal Field Flow Fraction 2773mentioning

confidence: 99%

On the Precision of Particle Size Analysis by Micro‐Thermal Field‐Flow Fractionation

Kašpárková

Halabalová

Šimek

et al. 2006

Journal of Liquid Chromatography & Related Technologies

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Micro-Thermal Field-Flow Fractionation of polymer colloidal particles was performed in two different laboratories. Short term repeatability of the experimental retentions obtained in a single laboratory has been found to be very high. The short term precision (expressed as percent standard deviation) of the determination of average particle diameter can reach the values greater than 1%, relative. Average repeatability of the retentions in both laboratories was better than 3%, relative when using identical experimental protocol. No other method of particle size analysis can provide the results of a comparable precision. Average repeatability of the width of the raw fractograms, which contains the information on particle size distribution, is of the order of 5%, relative. However, this value cannot be considered as the ultimate limit because the experiments were not carried at the low flow rate of the carrier liquid permitted to reach much higher resolution. The effect of the stability of the most important operational variables, such as the temperature drop between the cold and hot walls, the temperature of the cold wall, and the flow rate of the carrier liquid, on the precision of the analytical results is discussed.

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Separation of particles in nonaqueous suspensions by thermal-electrical field-flow fractionation

Cited by 38 publications

References 39 publications

Hyphenated thermal field flow fractionation–capillary electrophoresis

Hyphenated thermal field flow fractionation–capillary electrophoresis

Field‐flow fractionation of proteins, polysaccharides, synthetic polymers, and supramolecular assemblies

On the Precision of Particle Size Analysis by Micro‐Thermal Field‐Flow Fractionation

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