Separation of peptide fragments of a protein kinase C substrate fused to a β-hairpin by capillary electrophoresis

Zigoneanu, Imola G.; Sims, Christopher E.; Allbritton, Nancy L.

doi:10.1007/s00216-015-9065-8

Cited by 7 publications

(6 citation statements)

References 44 publications

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“…Peptidomics can also be used to monitor inter-individual divergence among cows [ 148 ] and as a tag for their health status [ 169 ]. Recently, a combination of CE, LC, like reversed-phase high-performance liquid chromatography (RP-HPLC), MS, and hydrophilic interacting liquid chromatography (HILIC) have been found to reveal bioactive peptides and their sequences in homogenized milk and human samples like sera, urine, and other intestinal substances [ 160 , 170 , 171 ].…”

Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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Food-based components represent major sources of functional bioactive compounds. Milk is a rich source of multiple bioactive peptides that not only help to fulfill consumers ‘nutritional requirements but also play a significant role in preventing several health disorders. Understanding the chemical composition of milk and its products is critical for producing consistent and high-quality dairy products and functional dairy ingredients. Over the last two decades, peptides have gained significant attention by scientific evidence for its beneficial health impacts besides their established nutrient value. Increasing awareness of essential milk proteins has facilitated the development of novel milk protein products that are progressively required for nutritional benefits. The need to better understand the beneficial effects of milk-protein derived peptides has, therefore, led to the development of analytical approaches for the isolation, separation and identification of bioactive peptides in complex dairy products. Continuous emphasis is on the biological function and nutritional characteristics of milk constituents using several powerful techniques, namely omics, model cell lines, gut microbiome analysis and imaging techniques. This review briefly describes the state-of-the-art approach of peptidomics and lipidomics profiling approaches for the identification and detection of milk-derived bioactive peptides while taking into account recent progress in their analysis and emphasizing the difficulty of analysis of these functional and endogenous peptides.

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Section: Advanced Analytical Techniques Of Milk and Dairy Productsmentioning

confidence: 99%

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

et al. 2020

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“…WKpG β‐hairpin peptide fused to a protein kinase C and its fragments generated by trypsin and pronase E were used as standards for identification of degradation fragments in a cellular lysate analyzed by CE with LIF detection . From the several acidic and basic buffers tested (100–300 mM phosphoric acid or phosphoric acid/sodium phosphate, pH 1.0–4.8; 170 mM sodium citrate, pH 4.8; 170 mM sodium acetate pH 4.0; 50 mM sodium phosphate, pH 8.8; 100 mM Tris, 100 mM Tricine, pH 8.1; 100 mM Tris, 100 mM tricine, 5 mM SDS; and 300 mM sodium borate, pH 7.5), the best separation was achieved within 16 min in 250 mM phosphoric acid, in 50 μm id capillary (30/20 cm total/effective lengths) at 6 kV separation voltage.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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“…Zigoneanu et al. developed a CE separation method for the WKpG β‐hairpin peptide and its proteolytic fragments in less than 16 min. The method has potential application for identification and quantification of WKpG‐PKC and its fragments in complex biological systems when the peptide is used as a reporter to assay protein kinase C (PKC) activity.…”

Section: Applicationsmentioning

confidence: 99%

Recent advances in microscale separation

et al. 2017

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Recent advances in microscale separationThis review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are cited covering the literature published

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Separation of peptide fragments of a protein kinase C substrate fused to a β-hairpin by capillary electrophoresis

Cited by 7 publications

References 44 publications

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent advances in microscale separation

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