Separation of phenolic compounds by centrifugal partition chromatography

Abstract

Phenolic acids are ubiquitous biomolecules exhibiting a wide range of physiological properties, with application in the pharmaceutical and nutraceutical fields. In this work, aqueous biphasic systems (ABS) formed by polyethylene glycol and sodium polyacrylate, and inorganic salts or ionic liquids as electrolytes, were applied to the purification of caffeic, ferulic and protocatechuic acids, followed by the use of centrifugal partition chromatography (CPC) to reinforce the fractionation process scale-up. In single-step experiments in ABS, high selectivities (SFA/CA = 12.09; SCA/PA = 6.32; SFA/PA = 1.91) and adequate partition coefficients (KCA = 2.78 ± 0.20; KPA = 0.44 ± 0.04; KFA = 0.23 ± 0.01) were achieved using ABS formed by sodium chloride as electrolyte. This system was further applied in CPC, allowing an efficient separation of the three phenolic acids after the optimization of the equipment operational conditions, while demonstrating the potential of polymer-based ABS to be used in liquid-liquid chromatography. Finally, the recovery of the phenolic acids (≥ 65%) with high purity from the ABS phases was demonstrated, followed by the reuse of the phase-forming components.

Comparison of two computational methods for solvent screening in countercurrent and centrifugal partition chromatography

“…The main advantages compared to solid-liquid chromatographic methods are well known, and include the use of lower amounts of solvents, no loss of components due to adsorption onto a solid support, lower maintenance costs, and the possibility of scaling up the process to pilot and industrial levels [ 4 , 5 ]. Both CPC and CCC techniques have been extensively applied to medicinal plant and natural product research [6] , from which relevant examples in the biorefinery context can be highlighted: the separation of monosaccharides from hydrolysed sugar beet pulp [7] , the fractionation of phenolic acids [8] , and the separation and purification of lignocellulosic biomass products (carbohydrates, furans, carboxylic acids and phenols) [9] . In this work, phenolics present in natural matrices are the target compounds; thus, vanillin (phenolic aldehyde), ferulic acid (hydroxycinnamic acid), ( S )-hesperetin (flavanone), and quercetin (flavonol) were selected as model compounds.…”

Fractionation of Isochrysis galbana Proteins, Arabinans, and Glucans Using Ionic-Liquid-Based Aqueous Biphasic Systems

“…41,65,66 Thus, the proposed approach appears as a promising, simple, and effective methodology of purification with industrial potential. Moreover, the use of IL-ABS can also be easily integrated into a fractionation process in continuous flow, through their posterior application on centrifugal partition chromatography 67 or continuous tubular separators. 68…”

Bioassay-Guided Isolation of Two Eudesmane Sesquiterpenes from Lindera strychnifolia Using Centrifugal Partition Chromatography

“…Molecules 2021, 26, 5269 2 of 10 operation mode, and the choice of a wide range of solvent systems [3][4][5][6][7][8][9][10][11][12], so it can achieve the fractionations with sufficient amount to further in vitro bioactivity tests [11,12]. Lindera strychnifolia Fernandez-Villar (Lauraceae) is an evergreen shrub that is widely distributed in Asia.…”

“…Centrifugal partition chromatography (CPC) is a type of support-free liquid-liquid chromatography technique that uses an immiscible two-phase solvent system to compose stationary and mobile phase. CPC offers many advantages such as a higher sample loading capacity, the absence of sample loss due to irreversible sample absorption to the solid column, a flexible operation mode, and the choice of a wide range of solvent systems [3][4][5][6][7][8][9][10][11][12], so it can achieve the fractionations with sufficient amount to further in vitro bioactivity tests [11,12]. Lindera strychnifolia Fernandez-Villar (Lauraceae) is an evergreen shrub that is widely distributed in Asia.…”