Separation of phenotypically indistinguishable Candida species, C. orthopsilosis, C. metapsilosis and C. parapsilosis, by capillary electromigration techniques

Candida “Psilosis” – electromigration techniques and MALDI-TOF mass spectrometry for phenotypical discrimination

“…35 The Candida strains were also separated in the narrow pH gradient (pH range from 3.6 to 4.0) and the pI values for C. parapsilosis strains were confirmed. 34 Furthermore, the newly determined pI values of C. orthopsilosis strains, biofilm-negative and biofilm-positive strains of C. metapsilosis are 3.83, 3.81 and 3.63, respectively.…”

“…31,32 In the capillary format, intact cells of Candida ''psilosis'' strains were characterized according to their electromigration properties by capillary zone electrophoresis (CZE) or capillary isoelectric focusing (CIEF). 33,34 CIEF of Candida ''psilosis'' strains in the narrow pH gradient has revealed that the isoelectric point (pI) for C. parapsilosis is 3.8 and 3.6 for biofilmnegative and biofilm-positive strains, respectively. 35 The Candida strains were also separated in the narrow pH gradient (pH range from 3.6 to 4.0) and the pI values for C. parapsilosis strains were confirmed.…”

Direct and Indirect Applications of Sub- and Supercritical Water in Food-Related Analysis

“…In order to appreciate the full range of prospective applications it is important to note that, because of amphoteric nature of cell membranes, even single-cell organisms such as bacteria display their effective surface charges and can therefore be separated by electromigration techniques including CIEF (Armstrong et al 1999;Shen et al 2000;Rodriguez and Armstrong 2004;Kremser et al 2007;Kostal and Arriaga 2008;Petr and Maier 2012;Š alplachta et al 2012). The applications of CIEF to this purpose in this laboratory have involved the separations of a large number of microbial species and strains related to food processing and protection (Horká et al 2009a) as well as to human health (Horká et al 2003(Horká et al , 2006a(Horká et al , b, c, 2009b(Horká et al , 2011Ruzicka et al 2007), e.g., separations of plant pathogens of the Clavibacter, Xanthomonas and Pseudomonas genera (Horká et al 2009a), separation of phenotypically indistinguishable Candida species (Horká et al 2011), differentiation between biofilm-positive and biofilm-negative Staphylococcus epidermidis strains (Ruzicka et al 2007) and separations of multiple microorganisms including Candida sp. (Horká et al 2003(Horká et al , 2006a(Horká et al , b, c, 2009b, Enterococcus faecalis (Horká et al 2003(Horká et al , 2006a, Escherichia coli (Horká et al 2003(Horká et al , 2006a(Horká et al , b, c, 2009b, Klebsiela pneumoniae (Horká et al 2006b), Proteus vulgaris (Horká et al 2006b), Saccharomyces cerevisiae (Horká et al 2006c), Staphylococcus aureus (Horká et al 2006a), Staphylococcus epidermidis (Horká et al 2003(Horká et al , 2006a(Horká et al , b, c, 2009b, Streptococcus agalactiae (Horká et al 2006a, b) and Stenotrophomonas maltophilia …”

Latest improvements in CIEF: From proteins to microorganisms

“…The possibility of CIEF with UV detection to distinguish between biofilm‐negative C. orthopsilosis and both biofilm‐negative and biofilm‐positive C. metapsilosis and C. parapsilosis was investigated . For this purpose, very narrow pH gradients were used and subsequently differences in p I values of biofilm‐negative and biofilm‐positive Candida species were determined below 0.03 p I units.…”