Separation of platelets by centrifugal elutriation

Persidsky, Maxim D.; Buchholz, W

doi:10.1016/0011-2240(77)90078-5

Cited by 2 publications

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“…During loading of blood, as described for separation of granulocytes [8], with rotor spinning, hemolysis was frequently observed as pink plasma in our early experiments [15]. When this occurred, no PRP was displaced by advancing flow of medium but there was a sharp rise in fluid line pressure and visible settling of RBC mass.…”

Section: Platelet Aggregationmentioning

confidence: 91%

Separation of platelet‐rich plasma by modified centrifugal elutriation

Persidsky

Ling

1982

J of Clinical Apheresis

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The Beckman centrifugal elutriation (CE) system is modified for the separation of platelet-rich plasma (PRP) from human and rabbit blood. The Beckman separation chamber is found inadequate for this purpose, and a new chamber of conical shape has been developed. Optimal flow rate for separating PRP from whole blood with the new chamber of 11.3 ml capacity is 3.5 ml/min while centrifuging at 2500 rpm or 700 g. Collection process takes about 4 minutes. This new process is based on the principle of centrifugal counterflow displacement and filtration, and is different from the CE process which is based on counterflow centrifugation and differential elution. About 90% of total platelets are recovered with this new process. The collected samples are free of leucocytes and contained only a few erythrocytes. Platelets collected by either differential centrifugation (DC) or new procedures are found to be similar in morphological characteristics, both being discoidal. Other characteristics such as aggregation response induced by ADP or epinephrine, serotonin-14C secretion, and survival of autologous platelets in rabbits are also found to be similar. However, ATP release induced by ADP is consistently higher from platelets prepared by our procedure than from those prepared by the DC procedure.

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Section: Platelet Aggregationmentioning

confidence: 91%

Separation of platelet‐rich plasma by modified centrifugal elutriation

Persidsky

Ling

1982

J of Clinical Apheresis

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Centrifugal elutriation (counterstreaming centrifugation) of cells

Pretlow

1979

Cell Biophysics

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Lindahl first described the separation of cells by velocity sedimentation utilizing a special technique (counterstreaming centrifugation) that was later modified slightly and renamed centrifugal elutriation. Centrifugal elutriation has been applied, with variable degrees of success, to the separation of hemopoietic cells, mouse tumor cells, testicular cells, and a variety of other specialized cells as well as cells in particular phases of the cell cycle. The capacity of the elutriator to separate large numbers of cells is its chief advantage. The purities of the separated cells have not been compared with the purities of cells separated by other methods in most cases; such comparisons would permit more sophisticated comparison of elutriation with other techniques for velocity sedimentation.

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Separation of platelets by centrifugal elutriation

Cited by 2 publications

References 0 publications

Separation of platelet‐rich plasma by modified centrifugal elutriation

Separation of platelet‐rich plasma by modified centrifugal elutriation

Centrifugal elutriation (counterstreaming centrifugation) of cells

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