Separation of polysaccharides from Spirulina platensis by HSCCC with ethanol-ammonium sulfate ATPS and their antioxidant activities

Wu, Xueyan; Li, Ruichang; Zhao, Yongjie; Liu, Yang

doi:10.1016/j.carbpol.2017.06.023

Cited by 78 publications

(44 citation statements)

References 30 publications

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“…As displayed in Figure 7b, no peaks were observed in the spectral range 260–280 nm. This signifies the non‐existence of protein and nucleic acids and further confirms the deproteinization efficiency of the ethanol/(NH 4 ) 2 SO 4 system as previously reported by (Wu et al, ). These results are line with the preliminary Bradford assay conducted as presented in Table .…”

Section: Resultssupporting

confidence: 89%

“…This is shown by the higher carbohydrate and lower protein content (%) of the DFu‐AD samples (APS 10 , APS 20 , APS 30 ) comparing with the non‐sonicated APS control sample. The results can be ascribed to an effective deproteinization using the ethanol/(NH 4 ) 2 SO 4 ATPS (Wu, Li, Zhao, & Liu, ), and a robust desalination process, that was effective in removing a substantial amount of salt into the dialysate which was replaced after every 30 min throughout the experimental time.…”

Section: Resultsmentioning

confidence: 99%

“…The preliminary characterization of the freeze-dried samples is displayed in Table 3. From the results, it was observed that (Wu, Li, Zhao, & Liu, 2017), and a robust desalination process, that was effective in removing a substantial amount of salt into the dialysate which was replaced after every 30 min throughout the experimental time.…”

Section: Primary Characterization Of Aps Polysaccharidesmentioning

confidence: 99%

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Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Chikari

Han

Wang

et al. 2020

J Food Process Engineering

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The study investigated the effect of dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and desalination on the yield, microstructure and antioxidant properties of Astragalus polysaccharides (APS). Extracts were desalinated for 10 (APS10), 20 (APS20), and 30 (APS30) minutes. A 28.40% experimental yield, well correlated to the response surface methodology predicted model was achieved. Thermodynamic parameters were non‐spontaneous, irreversible and endothermic. Moreover, ultrasound enhanced the desalination rate and did not alter the chemical structure in the APS. Fourier Transform Infrared Spectroscopy showed a similar spectrum in all the APS samples. High‐performance gel chromatography and scanning electron microscopy, respectively presented reduced molecular weights and altered microstructures in the samples. Monosaccharide analysis revealed the existence of xylose, mannose, galactose, glucose, arabinose, rhamnose, and ribose in the APS samples. Moreover, the APS20 showed strong hydroxyl and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activities, thus showing its potency in reducing oxidative stress. Hence, this study demonstrates that coupled ultrasound and alcohol/salt ATPS is a sustainable technique, which generates high yields of simultaneously extracted products. Practical applications Astragalus root has been used as an effective complementary treatment to conventional medicine owing to its rich nutraceutical composition and its numerous health attributes. Commercialization of Astragalus supplements into functional foods and nutraceuticals requires continuous exploration of novel, low cost and reproducible methods that retain their bioactivity. In this work, a synergized and bio‐compatible dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase system was used to extract and purify Astragalus polysaccharides. Application of this technique in the nutraceutical industry reduces treatment time, lowers bulk energy consumption hence reducing energy costs. It promotes the use of low‐cost, non‐hazardous bio‐based solvents in treatment processes thus considerably reducing extraction costs and environmental degradation. Findings from this study exhibit the practicability of this technique in industrial application. It equips biotechnologists with information on improved, sustainable and safer ways of extracting and enriching the quality of nutraceutical compounds.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Primary Characterization Of Aps Polysaccharidesmentioning

confidence: 99%

See 1 more Smart Citation

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Chikari

Han

Wang

et al. 2020

J Food Process Engineering

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“…The use of counter current chromatography systems employing ATPS has been reported as a strategy to enhance the recovery yields of proteins with good results and as an introduction to continuous mode operation . The purification of polysaccharides from Spirulina platensis using one‐step high speed counter current chromatography (HSCCC) with ethanol‐ammonium sulfate ATPS has been recently addressed . This is a different kind of continuous process that allowed reaching a yield nearly five times higher than traditional column chromatography separation methods.…”

Section: Implementation Of Continuous Atps Processesmentioning

confidence: 99%

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

González‐Valdez

Mayolo‐Deloisa

Rito‐Palomares

2017

J of Chemical Tech & Biotech

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Traditionally, aqueous two-phase systems (ATPS) have been used as a liquid-liquid extraction technique for the primary recovery and purification of biological samples. The enormous potential of their usage comes with great economical and technical advantages mainly due to the mild physicochemical environment. Nowadays, the use of ATPS as a bioengineering technique is approaching an era where new possibilities are being explored to maximize their use and implementation in the development of novel practical applications and tools. In this context, 'intelligent' polymers are being used as phase forming chemicals in ATPS in route to process integration. Extractive fermentation in ATPS is being re-evaluated with the aim of effectively growing microorganisms while recovering their fermentation products in different phases. ATPS are also being used as a tool for refolding of proteins. There are also several innovative efforts being made towards implementing this bioengineering tool as a continuous process moving away from traditional batch operations. In general, the possibilities of implementing ATPS in different bioprocessing aspects are growing considerably and gaining importance. This review aims to present the novel trends in the use and development of ATPS strategies as complete bioengineering tools and to provide a full perspective of their possibilities in the near future.

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“…All these modes made it possible for efficient separation of polar compounds by HSCCC, although it is still regarded as a main research challenge due to small number of theoretical plates. Several studies were conducted on separation of polar compounds by HSCCC as well .…”

Section: Introductionmentioning

confidence: 99%

Separation of three polar compounds from Rheum tanguticum by high‐speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system

Chen

Wang²,

Wang

et al. 2018

J of Separation Science

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The separation of polar compounds by high-speed countercurrent chromatography is still regarded as a challenge. In this study, an efficient strategy for the separation of three polar compounds from Rheum tanguticum has been successfully conducted by using high-speed countercurrent chromatography. X-5 macroporous resin chromatography was used for the fast enrichment of the target compounds. Then, the target fraction was directly introduced into high-speed countercurrent chromatography for separation using ethyl acetate/glacial acetic acid/water (100:1:100, v/v/v) as the solvent system. Consequently, three polar compounds including gallic acid, catechin, and gallic acid 4-O-β-d-(6'-O-galloyl) glucoside were obtained with purities higher than 98%. The results showed glacial acetic acid could be such an appropriate regulator for the ethyl acetate/water system. This study provides a reference for the separation of polar compounds from natural products by high-speed countercurrent chromatography.

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Separation of polysaccharides from Spirulina platensis by HSCCC with ethanol-ammonium sulfate ATPS and their antioxidant activities

Cited by 78 publications

References 30 publications

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Novel aspects and future trends in the use of aqueous two‐phase systems as a bioengineering tool

Separation of three polar compounds from Rheum tanguticum by high‐speed countercurrent chromatography with an ethyl acetate/glacial acetic acid/water system

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