Separation of polystyrene nanoparticles bearing different carboxyl group densities and functional groups quantification with capillary electrophoresis and asymmetrical flow field flow fractionation

You, Zengchao; Nirmalananthan-Budau, Nithiya; Resch‐Genger, Ute; Panne, Ulrich; Weidner, Steffen M.

doi:10.1016/j.chroma.2020.461392

Cited by 10 publications

(11 citation statements)

References 36 publications

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“…In general, the characterization of particle surface charge is carried out on fractions collected after AF4 separation. As an example, the offline hyphenation of AF4 and capillary electrophoresis has been applied to differentiate plain and carboxylated polystyrene nanoparticles [100,101].…”

Section: Particle Surface Chargementioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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The importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior. Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials. Despite this potential, the practical information that can be retrieved by AF4 and its possible applications are still rather unfamiliar to the pharmaceutical scientist. This review was conceived as a primer that clearly states the “do’s and don’ts” about AF4 applied to the characterization of polymeric nanocarriers. Aside from size characterization, AF4 can be beneficial during formulation optimization, for drug loading and drug release determination and for the study of interactions among biomaterials. It will focus mainly on the advances made in the last 5 years, as well as indicating the problematics on the consensus, which have not been reached yet. Methodological recommendations for several case studies will be also included. Graphical abstract

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Section: Particle Surface Chargementioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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“…In aqueous media, electrostatic and/or steric repulsion are responsible for the colloidal stability of nanoparticles. , The former correlates with the zeta potential . Typical values for nanoparticles stabilized in aqueous media by carboxylic groups are between −20 and −40 mV.…”

Section: Resultsmentioning

confidence: 99%

“…33,74 The former correlates with the zeta potential. 75 Typical values for nanoparticles stabilized in aqueous media by carboxylic groups are between −20 and −40 mV. Tamang et al have shown that for binary InP/ZnS QDs using mono-and bidentate thiol ligands the ligand-to-QD ratio can have a significant effect on colloidal stability after phase transfer.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Influence of Hydrophilic Thiol Ligands of Varying Denticity on the Luminescence Properties and Colloidal Stability of Quaternary Semiconductor Nanocrystals

et al. 2022

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Binary photoluminescent semiconductor nanocrystals (quantum dots, QDs) are one of the best studied fluorescent nanomaterials, and their unique optoelectronic properties paved the road to many applications in (bio)nanophotonics, optoelectronics, and photovoltaics. However, concerns related to their toxic constituents like cadmium or lead and the emerging interest in greener chemistry synthesis approaches hamper their future applicability. Interesting alternatives for some applications like biosensing or bioimaging are heavy-metal-free ternary QDs like AgInS2 (AIS), CuInS2 (CIS), and quaternary QDs such as AIS-ZnS (ZAIS). In this context, we explored the effect of ligand denticity on the organic-to-aqueous phase transfer of oleylamine-stabilized ZAIS QDs with the hydrophilic ligands mercaptopropionic acid (MPA), dihydrolipoic acid (DHLA), and 3-mercapto-2,2-bis(mercaptomethyl)propanoic acid (3MPA), bearing mono-, bi-, and trialkyl thiol groups. Spectroscopic studies of the resulting water-dispersible ZAIS QDs revealed a considerable influence of ligand denticity and ligand-to-QD ratio on the spectral position and width (FWHM; full width at half-maximum) of the photoluminescence (PL) bands, the PL quantum yields (PL QY), and the PL decay kinetics. Thiol capping and phase transfer resulted in a loss in PL by at least a factor of 2. The ligand-induced PL quenching observed particularly for ligands bearing two or three thiol groups was attributed to the facilitated formation of surface-bound disulfides. The best colloidal stability under high dilution conditions was observed for 3MPA.

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“…Combining AF4 and CE provides separation by size and surface charge. The correlation of these parameters requires a calibration curve with similarly sized particles of known FG density to correlate the CE retention times with FG density [ 258 ]. This method is, however, only suited for surface FG that control particle charge, e.g., for (de)protonable functionalities such as carboxylic acids and amines.…”

Section: Other Methods For Fg Quantification On Nanomaterialsmentioning

confidence: 99%

Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands

et al. 2021

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Functional nanomaterials (NM) of different size, shape, chemical composition, and surface chemistry are of increasing relevance for many key technologies of the twenty-first century. This includes polymer and silica or silica-coated nanoparticles (NP) with covalently bound surface groups, semiconductor quantum dots (QD), metal and metal oxide NP, and lanthanide-based NP with coordinatively or electrostatically bound ligands, as well as surface-coated nanostructures like micellar encapsulated NP. The surface chemistry can significantly affect the physicochemical properties of NM, their charge, their processability and performance, as well as their impact on human health and the environment. Thus, analytical methods for the characterization of NM surface chemistry regarding chemical identification, quantification, and accessibility of functional groups (FG) and surface ligands bearing such FG are of increasing importance for quality control of NM synthesis up to nanosafety. Here, we provide an overview of analytical methods for FG analysis and quantification with special emphasis on bioanalytically relevant FG broadly utilized for the covalent attachment of biomolecules like proteins, peptides, and oligonucleotides and address method- and material-related challenges and limitations. Analytical techniques reviewed include electrochemical titration methods, optical assays, nuclear magnetic resonance and vibrational spectroscopy, as well as X-ray based and thermal analysis methods, covering the last 5–10 years. Criteria for method classification and evaluation include the need for a signal-generating label, provision of either the total or derivatizable number of FG, need for expensive instrumentation, and suitability for process and production control during NM synthesis and functionalization. Graphical abstract

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Separation of polystyrene nanoparticles bearing different carboxyl group densities and functional groups quantification with capillary electrophoresis and asymmetrical flow field flow fractionation

Cited by 10 publications

References 36 publications

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Influence of Hydrophilic Thiol Ligands of Varying Denticity on the Luminescence Properties and Colloidal Stability of Quaternary Semiconductor Nanocrystals

Analyzing the surface of functional nanomaterials—how to quantify the total and derivatizable number of functional groups and ligands

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