Separation of prostaglandins A, B and C by thin-layer chromatography on ferric chloride impregnated silica gel

Wickramasinghe, J. A. F.; Shaw, Sophie

doi:10.1016/0090-6980(73)90125-1

Cited by 19 publications

(2 citation statements)

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“…This fraction recurred on extraction and rechromatography of the PGA1-3H zone, and therefore appeared to originate during chromatography. Thin-layer Chromatography on ferric chlorideimpregnated silica gel (a procedure which separates PGA, PGB and PGC; Wickramasinghe & Shaw, 1973), showed that on sequential rechromatography 0.7 to 2.7% of the radioactivity was associated with the PGB, zone. Since this fraction recurred on rechromatography of the extracted PGA, zone, it represents the extent of formation of PGB, from PGA, during chromatography and/or subsequent extraction.…”

Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

“…The purity was established by t.1.c. on: (a) untreated, precoated silica gel plates in ethyl acetate-acetic acid-2,2,4-trimethyl pentane-water (9 : 2 : 5 : 10) (organic phase), and (b) on ferric-chloride-impregnated silica gel plates in ethyl acetate-hexane-acetic acid (30 : 19 : 1) (Wickramasinghe & Shaw, 1973). Animals.…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

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The fate of prostaglandin A1-5,6-3H in the rat

Sinha

Shaw

1977

Journal of Pharmacy and Pharmacology

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The plasma concentrations, tissue distribution and excretion of prostaglandin A1 (PGA1) and related metabolites have been determined in rats, following the intravenous injection of a single dose of PGA1‐5,6‐3H. Urinary and faecal excretion accounted for averages of 25 and 43%, of the administered dose of PGA1, respectively. Oxidative cleavage of the carboxyl side chain of PGA1 appeared to be a major metabolic pathway in the rat. PGA1‐5,6‐3H was deemed unsuitable for metabolism studies in man, in view of the significant loss of tritium label from the prostaglandin.

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Section: R E S U L T S a N D Discussionmentioning

confidence: 99%

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

The fate of prostaglandin A1-5,6-3H in the rat

Sinha

Shaw

1977

Journal of Pharmacy and Pharmacology

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Radioimmunoassay of 11α, 15‐Dihydroxy‐9‐Keto‐Prosta‐5, 13‐Dienoic Acid (Prostaglandin e₂) and 9α, 11α, 15‐Trihydroxyprosta‐5, 13‐dienoic acid (prostaglandin f_2α) in human plasma

Lijnen

Verschueren

Amery

1981

Bulletin des Soc Chimique

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Tritium labeled diflorasone diacetate

Ayer

1978

Labelled Comp Radiopharmac

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SUMMARYD i f l o r a s o n e diacetate* (111) i s c a t a l y t i c a l l y reduced w i t h t r i t i u m gas t o g i v e t h e A-ring saturated 1 , 2 , 4 , 5 -t e t r a t r i t i a t e d compound. The double bonds i n t h e 1,2-and 4,5-positions a r e r e s t o r e d by dehydrogena t i on w i t h 2,3-di c h l oro-5,6-di cyano-l,4-benzoqui none (DDQ) . t r i t i u m l a b e l e d d i f l o r a s o n e d i a c e t a t e thus prepared has a s p e c i f i c a c t i v i t y o f 7.69 Ci/mM a f t e r chromatographic p u r i f i c a t i o n . t r i t i u m r e t e n t i o n i n d i c a t e s t h a t the i n t r o d u c t i o n o f t r i t i u m d u r i n g r e d u c t i o n and i t s removal TheThe low

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Separation of prostaglandins A, B and C by thin-layer chromatography on ferric chloride impregnated silica gel

Cited by 19 publications

References 8 publications

The fate of prostaglandin A1-5,6-3H in the rat

The fate of prostaglandin A1-5,6-3H in the rat

Radioimmunoassay of 11α, 15‐Dihydroxy‐9‐Keto‐Prosta‐5, 13‐Dienoic Acid (Prostaglandin e₂) and 9α, 11α, 15‐Trihydroxyprosta‐5, 13‐dienoic acid (prostaglandin f_2α) in human plasma

Tritium labeled diflorasone diacetate

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Separation of prostaglandins A, B and C by thin-layer chromatography on ferric chloride impregnated silica gel

Cited by 19 publications

References 8 publications

The fate of prostaglandin A1-5,6-3H in the rat

The fate of prostaglandin A1-5,6-3H in the rat

Radioimmunoassay of 11α, 15‐Dihydroxy‐9‐Keto‐Prosta‐5, 13‐Dienoic Acid (Prostaglandin e2) and 9α, 11α, 15‐Trihydroxyprosta‐5, 13‐dienoic acid (prostaglandin f2α) in human plasma

Tritium labeled diflorasone diacetate

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Product

Resources

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Radioimmunoassay of 11α, 15‐Dihydroxy‐9‐Keto‐Prosta‐5, 13‐Dienoic Acid (Prostaglandin e₂) and 9α, 11α, 15‐Trihydroxyprosta‐5, 13‐dienoic acid (prostaglandin f_2α) in human plasma