Separation of Soluble Brucella Antigens by Gel-Filtration Chromatography

Abstract

Soluble precipitating antigens of Brucella suis have been, in various degrees, purified by filtration on Sephadex gels. The most useful gels employed were Sephadex G-150, Sephadex G-200, and Sepharose 4B. Although not all fractions proved to be immunologically pure, some crude molecular-size estimates of most of the 13 soluble antigens of the Brucella cell could be given. In addition, monospecific antisera to three purified Brucella antigens have been prepared. By using purified preparations, physical and chemical data were obtained on two major antigens, E and 1, and a minor antigen, f. Antigen E is not an agglutinogen and may be toxic. Antigen I is of low molecular weight and is neither toxic nor agglutinogenic. The minor antigen f is an agglutinogen as well as a precipitinogen and is found on the cell surface. Both major antigens, when purified, were immunogenic in rabbits. Although soluble Brucella antigens have beenon August 1, 2020 by guest http://iai.asm.org/ Downloaded from

Διάγνωσις Της Βρουκελλώσεως Του Ανθρώπου Διά Της Ανοσοενζυματικής Μεθόδου (ELISA)

“…ΑΙ ούσίαι αύται ήσαν φωσφορολιποπολυσακχαροπρωτεϊνικής φύσεως ή λιπο-πολυσακχαριδικής. Ή ακριβής συσχέτισις τών ουσιών τούτων προς τα αντιγόνα Α καί Μ δέν είναι βεβαία (Hinsdill and Berman 1967, Diaz et al 1968, McGhee and Freeman 1970.…”