Separation of some anthocyanidins, anthocyanins, proanthocyanidins and related substances by reversed-phase high-performance liquid chromatography

Casteele, Karel Vande; Geiger, Hans; Loose, R. De; Sumere, C. F. Van

doi:10.1016/s0021-9673(01)88009-2

Cited by 60 publications

(26 citation statements)

References 22 publications

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“…Although the RP-HPLC separation of anthocyanins and flavonoids has been studied earlier and reviewed (Vande Casteele et al, 1982: Van Sumere et al, 1985aMarkham, 1989;Strack and Wray, 1989) a new RP-HPLC system was developed for an optimal separation of the flavonoids and phenolics which may occur in Rosa petals ( Fig. 1; Table 2).…”

Section: Resultsmentioning

confidence: 99%

“…For the above reasons it is not surprising that acceptable extraction methods and reversed phase-high performance liquid chromatographic (RP-HPLC) techniques for the separation of anthocyanins and other accompanying flavonoids have been elaborated (Vande Casteele et al, 1982Asen, 1982;Asen and Griesbach, 1983;Van Sumere ef af., 1985a,b). Moreover, combinations of these methodologies have been applied to the 'Flavonoid RP-HPLC Fingerprinting' of the petals of a series of ornamentals (Strack et al, 1980;Asen, 1982; Asen and Griesbach, C .…”

Section: Introductionmentioning

confidence: 99%

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Improved extraction and reversed phase‐high performance liquid chromatographic separation of flavonoids and the identification of Rosa cultivars

Sumere

Faché

Casteele

et al. 1993

Phytochemical Analysis

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Optimal extraction methods and Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) procedures were developed for the isolation and resolution of naturally occurring flavonoids and related phenolic compounds in Rosa petals. The following flavonoids and related phenolics were identified or partly identified: cyanidin 3,5-di-O-glucoside; cyanidin 3,s-di-0-glycoside (sugar moieties, glucose and arabinose); pelargonidin 3,s-di-0-glycoside; pelargonidin 3-0-glycoside; quercetin 3-0-glucoside; ellagic acid; quercetin 3-0-arabinoside; quercetin 4'-O-glucoside; kaempferol 4'-O-glucoside; kaempferol 3-0-rutinoside; kaempferol 3-0-glucoside; kaempferol3-0-arabinoside; kaempferol3-0-rhamnoside and kaempferol3-0-glycoside. Significant qualitative and quantitative differences between the above flavonoid markers allowed an objective distinction between 44 cultivars examined. The production of 'Flower Flavonoid RP-HPLC Fingerprints', which have been converted in the corresponding 'Computer Flower Flavonoid Identity Cards' via a suitable computer program, has proven to be of great value for Rosa cultivar recognition.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved extraction and reversed phase‐high performance liquid chromatographic separation of flavonoids and the identification of Rosa cultivars

Sumere

Faché

Casteele

et al. 1993

Phytochemical Analysis

Self Cite

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“…The elution gradient used at a flow rate of 1.7 ml/min consisted of A: HPLC‐grade methanol and B: 0.3% ( v / v ) perchloric acid in Millipore ultrapure water. The gradient elution program was the following: 0–2 min, isocratic 7% A; 2–8 min, linear gradient to 15% A; 8–25 min, linear gradient to 75% A; 25–27 min, linear gradient to 80% A; 27–29 min, linear to 100% A; and 29–40 min, isocratic 100% A …”

Section: Methodsmentioning

confidence: 99%

Combining SERS and microspectrofluorimetry with historically accurate reconstructions for the characterization of lac dye paints in medieval manuscript illuminations

Castro

Pozzi

Leona

et al. 2014

J Raman Spectroscopy

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In the present study, a number of dark red microsamples from nine illuminated manuscripts of three important medieval Portuguese monasteries (St. Mamede of Lorvão, Holy Cross of Coimbra and St. Mary of Alcobaça) were analyzed using a multi-analytical approach to identify the dyes, fillers, binders and other additional paint components. Historically accurate reconstructions of lac dye paints were prepared according to recipes from medieval treatises and characterized as reference materials. Its purpose was to create better means to study this complex dye, by using the materials and techniques as close as possible to the medieval ones and ultimately build a solid database to support the interpretation of the results obtained from the spectroscopic techniques. Surface-enhanced Raman spectroscopy (SERS) and microspectrofluorimetry were used here for the first time as complementary techniques to characterize lac dye paints, whereas Raman microscopy and micro-Fourier transform infrared spectroscopy were employed to detect binders and fillers. While SERS was able to offer a conclusive molecular fingerprint of lac dye, microspectrofluorimetry provided useful information on the global formulation of the red paints.

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“…Qualitative analyses are often carried out using TLC on silica gel (37,(40)(41). HPLC on reversedphase material offers better separation and is a commonly applied method for qualitative analysis of procyanidins (for examples, see [42][43][44][45], but, nevertheless, the reported chromatograms lack a selective baseline separation and reveal remarkable matrix effects.…”

Section: Previous Analytical Work With Crataegusmentioning

confidence: 99%

Hawthorn (Crataegus): Biological Activity and New Strategies for Quality Control

Sticher¹,

Meier²

1998

ACS Symposium Series

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Separation of some anthocyanidins, anthocyanins, proanthocyanidins and related substances by reversed-phase high-performance liquid chromatography

Cited by 60 publications

References 22 publications

Improved extraction and reversed phase‐high performance liquid chromatographic separation of flavonoids and the identification of Rosa cultivars

Improved extraction and reversed phase‐high performance liquid chromatographic separation of flavonoids and the identification of Rosa cultivars

Combining SERS and microspectrofluorimetry with historically accurate reconstructions for the characterization of lac dye paints in medieval manuscript illuminations

Hawthorn (Crataegus): Biological Activity and New Strategies for Quality Control

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