Separation of Testosterone and Dihydrotestosterone in Semen by High Performance Liquid Chromatography

Yang, H. Z.; Miao, Zhu-Lin; He, Zhu; Ji, Simin; Zeng, F.

doi:10.1080/01483918808076793

Journal of Liquid Chromatography

1988

DOI: 10.1080/01483918808076793

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Separation of Testosterone and Dihydrotestosterone in Semen by High Performance Liquid Chromatography

H. Z. Yang

Zhu-Lin Miao

Zhu He

et al.

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“…The assay was terminated by the addition of 10 μl 2.8 M NaOH. Educt ( 3 H-testosterone) and products ( 3 H-DHT, 3H-alpha-adiol and other metabolites) were separated by HPLC from each other using an adapted[22] and validated method, and using a Beckman System Gold HPLC (Beckman Instruments, San Ramon, CA, USA) fitted with an Onyx mo-…”

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confidence: 99%

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>

Heim¹,

Seibt²,

Stier³

et al. 2018

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Dihydrotestosterone (DHT) is implicated in the development of benign prostate hyperplasia (BPH). We investigated if Uromedic® pumpkin (variety of Cucurbita pepo L. convar. citrullinina GREB. var. styriaca GREB) seed soft extract (active ingredients of GRANUFINK® Prosta forte 500 mg), seed oil and isolated Δ7-sterols could inhibit the conversion of [1,2,6,7-3 H(N)]-testosterone to DHT by 5α-reductases. Also, we tested competition with [ 3 H]-DHT for binding to the androgen receptor (AR). Pumpkin seed oil and pumpkin seed soft extract were identified as moderately active inhibitors of 5α-R1 and 5α-R2, with almost similar inhibitory capacities (IC 50 < 5 mg/ml for 5α-R1 and about IC 50 = 6 mg/ml for 5α-R2). The isolated Δ7-sterols were more active inhibitors (IC 50 = 0.3 mg/ml for 5α-R1, IC 50 = 1.0 mg/ml for 5α-R2). All three test compounds bound to the AR dose-dependently, with strong binding by Δ7-sterols (IC 50 = 0.2 mg/ml) and weaker binding by pumpkin seed oil (IC 50 = 0.4 mg/ml) and pumpkin seed soft extract (IC 50 = 1.1 mg/ml). We propose that inhibition of 5α-reductases and competitive binding to the AR are mechanisms of action, by which the Uromedic® pumpkin seed derived test compounds, most specifically Δ7-sterols, counteract DHT and thereby exert clinically positive effects on the prostate, as well as bladder-strengthening effects.

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mentioning

confidence: 99%

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>

Heim¹,

Seibt²,

Stier³

et al. 2018

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Separation of Testosterone and Dihydrotestosterone in Semen by High Performance Liquid Chromatography

Cited by 1 publication

References 11 publications

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>

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Separation of Testosterone and Dihydrotestosterone in Semen by High Performance Liquid Chromatography

Cited by 1 publication

References 11 publications

Uromedic&#174; Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor &lt;em&gt;in Vitro&lt;/em&gt;

Uromedic&#174; Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor &lt;em&gt;in Vitro&lt;/em&gt;

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About

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>

Uromedic® Pumpkin Seed Derived Δ7-Sterols, Extract and Oil Inhibit 5α-Reductases and Bind to Androgen Receptor <em>in Vitro</em>