Separation of tetracycline and its related substances: comparison of liquid chromatography, capillary electrophoresis and capillary electrochromatography

Stationary and mobile phases in capillary electrochromatography (CEC)This review describes the state of the art of capillary electrochromatography (CEC). Properties of and interactions between stationary and mobile phases applied in CEC are described and discussed; developments in stationary phases, partly also monolithic stationary phases, are reviewed. Special attention is paid to the comparison of the behaviour of stationary and/or mobile phases under CEC versus HPLC conditions with respect to variables such as particle and pore size of the stationary phase, mobile phase composition, and temperature. These issues are discussed throughout the paper. A number of applications in CEC is presented as well.

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Isolation and identification of two unknown impurities from the raw material of clindamycin hydrochloride

Sun

Qin³

2014

J. Sep. Science

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Clindamycin hydrochloride belongs to the antibiotic family of lincomycin. It has the same antibacterial spectrum as lincomycin, but the antibacterial activity is four to eight times stronger than that of lincomycin. There have been some adverse reactions in clinical use of clindamycin hydrochloride and its finished drug products. The impurities in drugs are directly related to their safety. In this study, two unknown impurities were isolated from the raw material of clindamycin hydrochloride through various chromatographic methods. Their structures were identified as clindamycin isomer (impurity 1) and dehydroclindamycin (impurity 2) by mass spectrometry and NMR spectroscopy. Both of them were found for the first time. The two impurities exhibit a similar but lower antibacterial activity compared with clindamycin hydrochloride.

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Separation of tetracycline and its related substances: comparison of liquid chromatography, capillary electrophoresis and capillary electrochromatography

Cited by 5 publications

References 3 publications

Separation of tetracycline and its potential impurities by liquid chromatography on XTerra RP-18

Separation of tetracycline and its potential impurities by liquid chromatography on XTerra RP-18

Stationary and mobile phases in capillary electrochromatography (CEC)

Isolation and identification of two unknown impurities from the raw material of clindamycin hydrochloride

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