Separation of the collagen protein by ultrafiltration: Effects of concentration on the membrane's characteristics

Abstract

Collagen is one of the predominant proteins found in all multicellular animals. In this paper, the performance of the ultrafiltration process (UF) for concentrating the collagen protein was studied by performing batch experiments using a membrane module with an effective area of 28 cm 2 and equipped with a regenerated cellulose membrane with a molecular weight cutoff of 5 kDa. The feed solution consisted of aqueous solutions of bovine collagen hydrolysate with a molecular weight of 10 kDa. The effects of feed concentration (1%, 2%, 3%, 4%, and 5%) on the performance were determined. The optimum operating conditions were: a transmembrane pressure of 5 bar and a temperature of 25 C. The results showed that the permeability decreases with the increase of the volumetric concentration factor (VCF) for all five concentrated solutions of collagen tested. Also, the rejection of proteins increases with the increase in the concentration of the collagen solutions. The study confirms that the clogging phenomenon was dependent on the increase of concentration. Further, a mathematical modeling of fouling mechanism was analyzed based on Hermia's model. The concentration of the collagen solutions was also highlighted by viscosity measurements.

Effect of concentration on virus removal for ultrafiltration membrane in drinking water production

“…The mechanisms leading to the relation between feed concentration and UF performance were also complex to understand. Few studies observed increase of retention with feed concentrations, but this phenomenon was associated with filtration flux decrease because of pore blocking [56][57][58], which was not consistent with the present study. Liu et al have shown a recovery enhancement of MS2 and ϕX174 phages with increasing feed concentrations [10].…”

Reactional Processes on Osmium–Polymeric Membranes for 5–Nitrobenzimidazole Reduction

“…The filming of the cellulose acetate solution in N-methylpyrolidine (NMP) was performed at room temperature and humidity, using a certain amount of 10% polymer solution by applying it directly to a smooth glass surface (intended for thin-layer chromatography) to a thickness of 500 μm. The immersion in the ethanol-water (EW) coagulation bath in a 1:2 volume ratio was performed after a predetermined time [53,54].…”

“…The Fourier Transform Infra-Red (FTIR) spectra obtained for the three composite membranes do not show differences in the position of the absorption bands compared to those of the base polymers [52][53][54].…”

Transport and Separation of the Silver Ion with n–decanol Liquid Membranes Based on 10–undecylenic Acid, 10–undecen–1–ol and Magnetic Nanoparticles

“…The same extraction efficiency can also be computed based upon the absorbance of the solutions [47][48][49][50], as in:…”