In this paper, we have studied the separation for the following biharmonic differential operator:is the space of all bounded linear operators on the Hilbert space H 1 and u is the biharmonic differential operator, while u = n i=1 ∂ 2 u ∂x 2 i is the Laplace operator in R n . Moreover, we have studied the existence and uniqueness of the solution of the biharmonic differential equationin the Hilbert space H , where f (x) ∈ H .